Multiple downy mildew effectors target the stress‐related NAC transcription factor Ls NAC 069 in lettuce
| Autor: | Meisrimler, Claudia‐nicole, Pelgrom, Alexandra J. E., Oud, Bart, Out, Suzan, Van Den Ackerveken, Guido, Plant Microbe Interactions, Sub Plant-Microbe Interactions |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine transcription factor relocalization Plant Science Endoplasmic Reticulum 01 natural sciences 03 medical and health sciences Bremia lactucae Protein Domains Stress Physiological Pseudomonas Arabidopsis Genetics NAC transcription factors Gene Silencing Transcription factor Phylogeny Disease Resistance Plant Diseases Pseudomonas cichorii Cell Nucleus biology Lactuca sativa Effector Endoplasmic reticulum drought stress Proteins Cell Biology Lettuce Biotic stress biology.organism_classification oomycetes Cell biology Protein Transport Transmembrane domain 030104 developmental biology Gene Expression Regulation Host-Pathogen Interactions Transcription Factors 010606 plant biology & botany |
| Zdroj: | Plant Journal, 99(6), 1098. Blackwell Publishing Ltd |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1111/tpj.14383 |
| Popis: | To cause disease in lettuce, the biotrophic oomycete Bremia lactucae secretes potential RxLR effector proteins. Here we report the discovery of an effector‐target hub consisting of four B. lactucae effectors and one lettuce protein target by a yeast‐two‐hybrid (Y2H) screening. Interaction of the lettuce tail‐anchored NAC transcription factor, LsNAC069, with B. lactucae effectors does not require the N‐terminal NAC domain but depends on the C‐terminal region including the transmembrane domain. Furthermore, in Y2H experiments, B. lactucae effectors interact with Arabidopsis and potato tail‐anchored NACs, suggesting that they are conserved effector targets. Transient expression of RxLR effector proteins BLR05 and BLR09 and their target LsNAC069 in planta revealed a predominant localization to the endoplasmic reticulum. Phytophthora capsici culture filtrate and polyethylene glycol treatment induced relocalization to the nucleus of a stabilized LsNAC069 protein, lacking the NAC‐domain (LsNAC069ΔNAC). Relocalization was significantly reduced in the presence of the Ser/Cys‐protease inhibitor TPCK indicating proteolytic cleavage of LsNAC069 allows for relocalization. Co‐expression of effectors with LsNAC069ΔNAC reduced its nuclear accumulation. Surprisingly, LsNAC069 silenced lettuce lines had decreased LsNAC069 transcript levels but did not show significantly altered susceptibility to B. lactucae. In contrast, LsNAC069 silencing increased resistance to Pseudomonas cichorii bacteria and reduced wilting effects under moderate drought stress, indicating a broad role of LsNAC069 in abiotic and biotic stress responses. |
| Databáze: | OpenAIRE |
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