Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations
| Autor: | David A. C. Beck, Katherine R. Heal, Nitin S. Baliga, Anitra E. Ingalls, Erik L. Hendrickson, E. Virginia Armbrust, Murray Hackett, Wei Qin, Andrew D. Holmes, Allan H. Devol, Sonia M. Tiquia-Arashiro, Neeraja Vajrala, Tony Wang, Fred Taub, Paul M. Berube, Todd M. Lowe, Luis A. Sayavedra-Soto, Shady A. Amin, Willm Martens-Habbena, Daniel J. Arp, Hidetoshi Urakawa, David A. Stahl, Serdar Turkarslan, Rachel A. Lundeen, James W. Moffett, Kyle C. Costa |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Proteomics Cobalamin biosynthesis Archaeal Proteins Nitrosopumilus Microbiology Carbon Cycle 03 medical and health sciences chemistry.chemical_compound Biosynthesis Ammonia Stress Physiological medicine Ecology Evolution Behavior and Systematics biology Carbon fixation Copper toxicity Ammonia monooxygenase biology.organism_classification medicine.disease Archaea Vitamin B 12 030104 developmental biology Biochemistry chemistry Original Article Oxidoreductases Transcriptome Water Microbiology Oxidation-Reduction Bacteria Copper |
| Popis: | High representation by ammonia-oxidizing archaea (AOA) in marine systems is consistent with their high affinity for ammonia, efficient carbon fixation, and copper (Cu)-centric respiratory system. However, little is known about their response to nutrient stress. We therefore used global transcriptional and proteomic analyses to characterize the response of a model AOA, Nitrosopumilus maritimus SCM1, to ammonia starvation, Cu limitation and Cu excess. Most predicted protein-coding genes were transcribed in exponentially growing cells, and of ~74% detected in the proteome, ~6% were modified by N-terminal acetylation. The general response to ammonia starvation and Cu stress was downregulation of genes for energy generation and biosynthesis. Cells rapidly depleted transcripts for the A and B subunits of ammonia monooxygenase (AMO) in response to ammonia starvation, yet retained relatively high levels of transcripts for the C subunit. Thus, similar to ammonia-oxidizing bacteria, selective retention of amoC transcripts during starvation appears important for subsequent recovery, and also suggests that AMO subunit transcript ratios could be used to assess the physiological status of marine populations. Unexpectedly, cobalamin biosynthesis was upregulated in response to both ammonia starvation and Cu stress, indicating the importance of this cofactor in retaining functional integrity during times of stress. |
| Databáze: | OpenAIRE |
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