Gas chromatographic–tandem mass spectrometric quantification of human plasma and urinary nitrate after its reduction to nitrite and derivatization to the pentafluorobenzyl derivative
| Autor: | Jürgen C. Frölich, Jörg Sandmann, Edzard Schwedhelm, Dimitrios Tsikas, Frank-Mathias Gutzki |
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| Rok vydání: | 1999 |
| Předmět: |
Chemical ionization
Nitrates Chromatography General Chemistry Tandem mass spectrometry Mass spectrometry Gas Chromatography-Mass Spectrometry Fluorobenzenes chemistry.chemical_compound chemistry Nitrate Humans Indicators and Reagents Gas chromatography Nitrite Derivatization Quantitative analysis (chemistry) Nitrites |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 731:285-291 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(99)00244-3 |
| Popis: | Gas chromatography-mass spectrometry (GC-MS) of nitrite as its pentafluorobenzyl derivative in the negative-ion chemical ionization mode is a useful analytical tool to quantify accurately and sensitively nitrite and nitrate after its reduction to nitrite in various biological fluids. In the present study we demonstrate the utility of GC-tandem MS to quantify nitrate in human plasma and urine. Our present results verify human plasma and urine levels of nitrite and nitrate measured previously by GC-MS. |
| Databáze: | OpenAIRE |
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