Direct action of light on serotonin metabolism and RNA biosynthesis in duck pineal explants
| Autor: | Carlos A. Nagle, JoséH. Denari, Greta Declercq de Pérez Bedés, Jorge M. Rosner, Daniel P. Cardinali, Liliana Orsi |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Serotonin medicine.medical_treatment Pinealectomy Dark Adaptation Biology Tritium Pineal Gland General Biochemistry Genetics and Molecular Biology Melatonin chemistry.chemical_compound Pineal gland Organ Culture Techniques Internal medicine medicine Animals Photoreceptor Cells General Pharmacology Toxicology and Pharmaceutics Uridine Cerebral Cortex Carbon Isotopes RNA General Medicine Metabolism Endocrinology medicine.anatomical_structure Ducks chemistry Organ Specificity Darkness Chromatography Thin Layer hormones hormone substitutes and hormone antagonists Photic Stimulation medicine.drug |
| Zdroj: | Life sciences. Pt. 2: Biochemistry, general and molecular biology. 11(17) |
| ISSN: | 0300-9637 |
| Popis: | In previous papers we have demonstrated 1) that pinealectomy diminishes significantly the stimulatory effects of light on the testicular metabolism of blinded ducks; and 2) that light has a direct effect on the hydroxyindole-O-methyl transferase activity of duck pineal explants. In the present paper we have further investigated the pineal photoreceptor function studying the direct action of light on the duck pineal gland, taking as parameters the metabolism of serotonin and the incorporation of [3H] uridine to RNA by pineal explants under different lighting conditions. Duck pineal and cerebral cortex explants were cultured for 24 hr under darkness or continuous light in TC 199 medium. Both the conversion of serotonin to methoxyindole acetic acid, hydroxytriptophol, methoxytriptophol, N-acetyl-serotonin and melatonin and the incorporation of [3H] uridine to RNA by duck pineal explants were significantly stimulated by light. Cerebral cortex explants utilized as a control tissue did not show significant differences in the incorporation of uridine under both lighting conditions. These results are a further evidence of the photoreceptor function of the duck pineal gland and indicate that light directly increases not only the pineal indole metabolism but also the incorporation of [3H] uridine to RNA. |
| Databáze: | OpenAIRE |
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