Neutralisation of the pharmacological activities of Bothrops alternatus venom by anti-PLA2 IgGs
| Autor: | Luis Alberto Ponce-Soto, Laura C. Leiva, Silvana Maruñak, Ofelia Acosta, Juan S. Todaro, María E. Garcia Denegri |
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| Rok vydání: | 2014 |
| Předmět: |
Male
ENVENOMATION Phospholipase A2 Inhibitors Myotoxin Venom Pharmacology Toxicology BOTHROPS ALTERNATUS Mice Phospholipase A2 Antigen Western blot Antibody Specificity Neutralization Tests Otras Ciencias Veterinarias Crotalid Venoms medicine Animals Bothrops NON-MYOTOXIC PLA2 Envenomation biology medicine.diagnostic_test Antivenins Ciencias Veterinarias biology.organism_classification Ouchterlony double immunodiffusion Antibodies Neutralizing Antibodies Anti-Idiotypic Bothrops alternatus Phospholipases A2 CIENCIAS AGRÍCOLAS Immunoglobulin G Immunology biology.protein lipids (amino acids peptides and proteins) Rabbits ANTI-PLA2 IMMUNOGLOBULINS |
| Zdroj: | Toxicon. 86:89-95 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/j.toxicon.2014.05.016 |
| Popis: | Basic phospholipases A2 (PLA2) are toxic and induce a wide spectrum of pharmacological effects, although the acidic enzyme types are not lethal or cause low lethality. Therefore, it is challenging to elucidate the mechanism of action of acidic phospholipases. This study used the acidic non-toxic Ba SpII RP4 PLA2 from Bothrops alternatus as an antigen to develop anti-PLA2 IgG antibodies in rabbits and used in vivo assays to examine the changes in crude venom when pre-incubated with these antibodies. Using Ouchterlony and western blot analyses on B. alternatus venom, we examined the specificity and sensitivity of phospholipase A2 recognition by the specific antibodies (anti-PLA2 IgG). Neutralisation assays using a non-toxic PLA2 antigen revealed unexpected results. The (indirect) haemolytic activity of whole venom was completely inhibited, and all catalytically active phospholipases A2 were blocked. Myotoxicity and lethality were reduced when the crude venom was pre-incubated with anti-PLA2 immunoglobulins. CK levels in the skeletal muscle were significantly reduced at 6 h, and the muscular damage was more significant at this time-point compared to 3 and 12 h. When four times the LD50 was used (224 µg), half the animals treated with the venom-anti PLA2 IgG mixture survived after 48 h. All assays performed with the specific antibodies revealed that Ba SpII RP4 PLA2 had a synergistic effect on whole-venom toxicity. IgG antibodies against the venom of the Argentinean species B. alternatus represent a valuable tool for elucidation of the roles of acidic PLA2 that appear to have purely digestive roles and for further studies on immunotherapy and snake envenoming in affected areas in Argentina and Brazil. Fil: Garcia Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Clinica. Catedra de Farmacologia; Argentina. Universidad Nacional del Nordeste. Facultad de Cs.exactas Naturales y Agrimensura. Departamento de Agrimensura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maruñak, Silvana L.. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina Fil: Todaro, Juan S.. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina Fil: Ponce Soto, Luis A.. Universidade Estadual Do Campinas. Instituto de Biologia; Brasil Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Leiva, Laura C.. Universidad Nacional del Nordeste. Facultad de Cs.exactas Naturales y Agrimensura. Departamento de Bioquimica. Laboratorio de Investigacion En Proteinas; Argentina |
| Databáze: | OpenAIRE |
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