Mechanosensitive currents in putative aortic baroreceptor neurons in vitro
| Autor: | Joseph Thomas Cunningham, Francois M. Abboud, Ruth E. Wachtel |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Osmosis
Baroreceptor Patch-Clamp Techniques Physiology Pressoreceptors In Vitro Techniques medicine.artery Physical Stimulation medicine Animals Patch clamp Reversal potential Evoked Potentials Aorta Neurons Voltage-dependent calcium channel Chemistry General Neuroscience Nodose Ganglion Rats nervous system Cell culture Biophysics Mechanosensitive channels |
| Zdroj: | Journal of neurophysiology. 73(5) |
| ISSN: | 0022-3077 |
| Popis: | 1. Whole cell patch-clamp experiments were conducted to determine whether rat aortic baroreceptor neurons contain mechano-sensitive conductances. 2. Putative aortic baroreceptor neurons in the nodose ganglia were identified by injecting DiI onto the adventitia of the aortic arch. Nodose ganglia neurons were dissociated after > or = 1 wk. A fluorescein-conjugated tetanus toxin fragment was used to confirm that the cells labeled with DiI in culture were neurons. 3. Hypoosmotic stretch significantly increased the conductance of DiI-labeled neurons (n = 19). The reversal potential of the response was -11 +/- 1 (SE) mV. 4. In experiments on unlabeled neurons, only 7 of 13 cells showed increases in conductance. BC3H1 cells, a mouse tumor cell line, showed no changes in conductance. 5. Gadolinium (20 microM), a putative blocker of mechanosensitive channels, prevented the increase in conductance produced by hypoosmolality in seven of seven labeled cells. Equimolar concentrations of lanthanum (n = 6) and omega-conotoxin GVIA (1 microM, n = 4), which block voltage-gated calcium channels, failed to significantly affect the inward current. |
| Databáze: | OpenAIRE |
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