Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of Their Sporozoites for Downstream Manipulation
| Autor: | Jon P. Boyle, Zhee Sheen Wong, Sarah L Sokol, Jitender P. Dubey |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
ved/biology 030106 microbiology ved/biology.organism_classification_rank.species Oocysts Toxoplasma gondii Biology biology.organism_classification Article Microbiology Hammondia hammondi Feces Mice 03 medical and health sciences 030104 developmental biology Sporozoites parasitic diseases Cats Animals Parasite hosting Toxoplasma |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493998562 Methods Mol Biol |
| DOI: | 10.1007/978-1-4939-9857-9_4 |
| Popis: | Toxoplasma gondii tachyzoites and bradyzoites are studied extensively in the laboratory due to the ease with which they can be cultured. In contrast oocysts and the sporozoites within them are more difficult to work with, in that cat infections are required for their generation and isolating sporozoites requires a laborious excystation procedure. More over some parasite species such as Hammondia hammondi are obligately heteroxenous and require passage through a cat for completion of the life cycle. There is no debate that there is great value in studying this important life cycle stage, and so we present here a detailed description of the current protocols used in our laboratories to generate and isolate T. gondii and H. hammondi oocysts, and to excyst and purify the sporozoites within them for use in downstream experimental applications. |
| Databáze: | OpenAIRE |
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