Mesenchymal and stemness circulating tumor cells in early breast cancer diagnosis
| Autor: | Michel Tartary, Guislaine Barrière, Alain Riouallon, Joël Renaudie, Michel Rigaud |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Oncology
CA15-3 Adult medicine.medical_specialty Cancer Research Epithelial-Mesenchymal Transition Breast Neoplasms lcsh:RC254-282 Aldehyde Dehydrogenase 1 Family Cohort Studies Circulating tumor cell Breast cancer Internal medicine Proto-Oncogene Proteins medicine Biomarkers Tumor Genetics Humans Epithelial–mesenchymal transition Lymph node Early Detection of Cancer Aged biology business.industry CD44 Twist-Related Protein 1 Bone metastasis Cancer Retinal Dehydrogenase Middle Aged medicine.disease Neoplastic Cells Circulating lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Isoenzymes medicine.anatomical_structure biology.protein Female Phosphatidylinositol 3-Kinase business Research Article |
| Zdroj: | BMC Cancer, Vol 12, Iss 1, p 114 (2012) BMC Cancer |
| ISSN: | 1471-2407 |
| DOI: | 10.1186/1471-2407-12-114 |
| Popis: | Background Epithelial mesenchymal transition (EMT) is a crucial event likely involved in dissemination of epithelial cancer cells. This process enables them to acquire migratory/invasive properties, contributing to tumor and metastatic spread. To know if this event is an early one in breast cancer, we developed a clinical trial. The aim of this protocol was to detect circulating tumor cells endowed with mesenchymal and/or stemness characteristics, at the time of initial diagnosis. Breast cancer patients (n = 61), without visceral or bone metastasis were enrolled and analysis of these dedifferentiated circulating tumor cells (ddCTC) was realized. Methods AdnaGen method was used for enrichment cell selection. Then, ddCTC were characterized by RT-PCR study of the following genes: PI3Kα, Akt-2, Twist1 (EMT markers) and ALDH1, Bmi1 and CD44 (stemness indicators). Results Among the studied primary breast cancer cohort, presence of ddCTC was detected in 39% of cases. This positivity is independant from tumor clinicopathological factors apart from the lymph node status. Conclusions Our data uniquely demonstrated that in vivo EMT occurs in the primary tumors and is associated with an enhanced ability of tumor cells to intravasate in the early phase of cancer disease. These results suggest that analysis of circulating tumor cells focused on cells showing mesenchymal or stemness characteristics might facilitate assessment of new drugs in clinical trials. |
| Databáze: | OpenAIRE |
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