The expression and characterization of endoglin in uterine leiomyosarcoma
| Autor: | Eiko Yamamoto, Takeshi Senga, Tomokazu Umezu, Hiroko Mitsui, Tomomi Kotani, Kiyosumi Shibata, Shiro Suzuki, Yukio Mano, Mika Mizuno, Fumitaka Kikkawa, Hiroaki Kajiyama |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Leiomyosarcoma Vascular Endothelial Growth Factor A Cancer Research medicine.medical_specialty Receptors Cell Surface Small hairpin RNA Antigens CD Transforming Growth Factor beta Cell Line Tumor hemic and lymphatic diseases Internal medicine otorhinolaryngologic diseases medicine Humans Neoplasm Invasiveness Extracellular Signal-Regulated MAP Kinases Aged biology business.industry Endoglin General Medicine Transfection Transforming growth factor beta Middle Aged medicine.disease Immunohistochemistry Vascular endothelial growth factor A Endocrinology Oncology Cell culture Uterine Neoplasms Cancer research biology.protein Female business Signal Transduction |
| Zdroj: | Clinical & Experimental Metastasis. 30:731-740 |
| ISSN: | 1573-7276 0262-0898 |
| DOI: | 10.1007/s10585-013-9574-9 |
| Popis: | Endoglin (CD105), an accessory receptor of transforming growth factor-β, is expressed in vascular endothelial cells. Recently, it was reported that endoglin expression was significantly associated with poorer survival in several cancers. In this study, we evaluated the role of endoglin in uterine leiomyosarcoma. We examined the expression of endoglin in 22 uterine leiomyosarcomas and the association between their expression and the outcome. Additionally, to evaluate the function of endoglin, we used SKN cells, a human uterine leiomyosarcoma cell line. We generated SKN cells stably transfected with plasmids encompassing shRNA targeting endoglin (shEng cells), and compared the ability of proliferation, migration, and invasion to control shRNA-transfected cells (shCon cells). We compared the level of VEGF and matrix metalloproteinases (MMP) in culture supernatants of shEndoglin and shControl cells. Nine patients were endoglin-positive and 13 patients were -negative. The endoglin-positive group had a significantly poorer overall survival and progression-free survival than the endoglin-negative group. In an in vitro study, there was no difference in cell proliferation between shEng and shCon cells. On the other hand, shEng cells showed a lower ability for migration and invasion than shControl cells. The activity of MMP-9 and VEGF level in the supernatant from shEng cells were lower than in shCon cells. In uterine leiomyosarcoma, endoglin expression was associated with a poor prognosis. It was suggested that endoglin up-regulated invasion and VEGF secretion. The investigation of endoglin may lead to a new strategy in uterine leiomyosarcoma therapy. |
| Databáze: | OpenAIRE |
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