Evaluation of chlorpyrifos effects, alone and combined with lipopolysaccharide stress, on DNA integrity and immune responses of the three-spined stickleback, Gasterosteus aculeatus
| Autor: | Patrick Baudoin, Adrien Marchand, Cyril Turies, Anne Bado-Nilles, Stéphane Betoulle, Olivier Palluel, Edith Chadili, Jean-Marc Porcher |
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| Přispěvatelé: | Institut National de l'Environnement Industriel et des Risques (INERIS), Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Erythrocytes DNA damage Three-spined stickleback Health Toxicology and Mutagenesis Context (language use) 010501 environmental sciences FISH : LIPOPOLYSACCHARIDES 01 natural sciences Microbiology Toxicology 03 medical and health sciences chemistry.chemical_compound Immune system Animals Ecosystem 0105 earth and related environmental sciences Innate immune system biology Public Health Environmental and Occupational Health Stickleback General Medicine biology.organism_classification Pollution Immunity Innate Smegmamorpha Respiratory burst Oxidative Stress 030104 developmental biology chemistry THREE-SPINED STICKLEBACK 13. Climate action Chlorpyrifos [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Water Pollutants Chemical DNA Damage |
| Zdroj: | Ecotoxicology and Environmental Safety Ecotoxicology and Environmental Safety, Elsevier, 2017, 145, pp.333-339. ⟨10.1016/j.ecoenv.2017.07.025⟩ |
| ISSN: | 1090-2414 0147-6513 |
| Popis: | Organism immune defences might be weakened by pollutants, largely detected in aquatic ecosystems, leading to the facilitation for opportunistic pathogens to infect organisms. In this context, destabilization of fish non-specific immune parameters and erythrocyte DNA integrity was tested, on a model fish species, the three-spined stickleback (Gasterosteus aculeatus), after exposure to chlorpyrifos (CPF). Alone, pesticide exposure induced a genotoxic potential (chlorpyrifos at 1.75 and 0.88 µg/L) in addition to a decrease in phagocytosis capacity and a stimulation of respiratory burst. Then, to mimic pathogenic infection, fish exposure to chlorpyrifos was combined with lipopolysaccharides (LPS) stress. In this second experiment, an increase of DNA damage was observed in fish exposed to a lower concentration of chlorpyrifos and LPS. Moreover, at the higher concentration of chlorpyrifos, an early destabilization of innate immunity was observed as suggested by the absence of an increase of lysosomal presence in fish injected with LPS. This study highlighted the usefulness of stress on stress responses to better understand the impact of contaminants on the organism's health. |
| Databáze: | OpenAIRE |
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