Inhibiting amyloid β-protein assembly: Size-activity relationships among grape seed-derived polyphenols
Autor: | Benson Chen, Jun Wang, Giulio Maria Pasinetti, Shiela Beroukhim, Eric Y. Hayden, Lin Jiang, David B. Teplow, Lap Ho, Mikhail Kibalchenko, Ghiam Yamin |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Models
Molecular Circular dichroism Molar concentration Protein Conformation Population amyloid -protein Biochemistry Oligomer Article Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Structure-Activity Relationship Models inhibitors Mole Structure–activity relationship Animals Vitis Benzothiazoles oligomers education polyphenols Fluorescent Dyes amyloid β-protein education.field_of_study Neurology & Neurosurgery Amyloid beta-Peptides Circular Dichroism Neurosciences Molecular Polyphenols Neurofibrillary Tangles Alzheimer's disease grape seeds Molecular Weight Thiazoles Cross-Linking Reagents chemistry Polyphenol Seeds Thioflavin Biochemistry and Cell Biology |
Zdroj: | Hayden, EY; Yamin, G; Beroukhim, S; Chen, B; Kibalchenko, M; Jiang, L; et al.(2015). Inhibiting amyloid β-protein assembly: Size-activity relationships among grape seed-derived polyphenols. Journal of Neurochemistry, 135(2), 416-430. doi: 10.1111/jnc.13270. UCLA: Retrieved from: http://www.escholarship.org/uc/item/84c655x1 Journal of Neurochemistry, vol 135, iss 2 Journal of neurochemistry, vol 135, iss 2 |
DOI: | 10.1111/jnc.13270. |
Popis: | © 2015 International Society for Neurochemistry. Epidemiological evidence that red wine consumption negatively correlates with risk of Alzheimer's disease has led to experimental studies demonstrating that grape seed extracts inhibit the aggregation and oligomerization of Aβ in vitro and ameliorate neuropathology and behavioral deficits in a mouse model of Alzheimer's disease. The active agent in the extracts is a mixed population of polyphenolic compounds. To evaluate the relative potency of each of these compounds, HPLC was used to fractionate the mixture into monomers, dimers, and oligomers. Each fraction was analyzed for its effect on Aβ conformational dynamics (circular dichroism), oligomerization (zero-length photochemical cross-linking), aggregation kinetics (Thioflavin T fluorescence), and morphology (electron microscopy). The relative activities of each fraction were determined on the basis of molar concentration (mol/L) or mass concentration (g/L). When molar concentration, the number concentration of each polyphenolic compound, was considered, the oligomer fraction was the most potent inhibitor of Aβ oligomerization and aggregation. However, when mass concentration, the number concentration of phenolic groups, was considered, monomers were the most potent inhibitors. To understand these ostensibly contradictory results, a model of polyphenol:Aβ complexation was developed. This model, which was found to be consistent with published X-ray crystallographic studies, offers an explanation for the effects of functional group polyvalency on inhibitor activity. Our data emphasize the importance of an in-depth understanding of the mechanism(s) underlying 'concentration dependence' in inhibitor systems involving polyfunctional agents. |
Databáze: | OpenAIRE |
Externí odkaz: |