Molecular mechanisms of methylsulfonylmethane and allicin in the inhibition of CD44 ± breast cancer cells growth
| Autor: | Maryam Dastan, Elham Sarkhani, Mohammad Mazani, Nasrin Tata, Nowruz Najafzadeh, Mohsen Arzanlou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Methylsulfonylmethane Breast carcinoma Medicine (miscellaneous) Apoptosis Cell cycle arrest 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine TX341-641 MTT assay CD44 Cytotoxicity Allicin Nutrition and Dietetics biology Nutrition. Foods and food supply Cell cycle Molecular biology 030104 developmental biology chemistry Cell culture 030220 oncology & carcinogenesis biology.protein Food Science |
| Zdroj: | Journal of Functional Foods, Vol 39, Iss, Pp 50-57 (2017) |
| ISSN: | 1756-4646 |
| DOI: | 10.1016/j.jff.2017.10.007 |
| Popis: | Breast carcinoma is a common cause of cancer death in women worldwide. CD44± cells were isolated from MCF7 cell line by magnetic-activated cell sorting (MACS) and treated with methylsulfonylmethane (MSM), allicin, and the combination of them. The cytotoxicity and cell cycle arrest were measured using MTT assay and flow cytometry. Moreover, mRNA levels of apoptosis regulators Bax, p53, and caspase-3 were measured using reverse transcriptase-polymerase chain reaction (RT-PCR). The combination treatment inhibited CD44− and CD44+ cells in the G2/M and S phases of the cell cycle, respectively. Importantly, Bax expression was significantly higher in the MSM/allicin-treated CD44+ cells than in the MSM- or allicin-treated cells (P |
| Databáze: | OpenAIRE |
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