Emergence and maintenance of multidrug-resistant Escherichia coli of canine origin harbouring a blaCMY-2-IncI1/ST65 plasmid and topoisomerase mutations
| Autor: | Eva Tzelepi, A. F. Koutinas (Α.Φ. Κουτινασ), Elpida I. Vingopoulou, M. N. Saridomichelakis, Victoria I. Siarkou, F. Kaltsogianni, G.C. Batzias, Vivi Miriagou, I. Tzavaras, Danai Sofianou, Effrosyni Sianou |
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| Rok vydání: | 2014 |
| Předmět: |
DNA Topoisomerase IV
Microbiology (medical) Salmonella Molecular Sequence Data Ceftazidime Microbial Sensitivity Tests Biology medicine.disease_cause beta-Lactam Resistance beta-Lactamases Dermatitis Atopic Microbiology Cefoxitin Feces Dogs Plasmid Bacterial Proteins Drug Resistance Multiple Bacterial Escherichia coli medicine Animals Otitis Pharmacology (medical) Typing Escherichia coli Infections Pharmacology Enrofloxacin Broth microdilution biochemical phenomena metabolism and nutrition Virology Anti-Bacterial Agents Bacterial Typing Techniques Cephalosporins Multiple drug resistance Infectious Diseases DNA Gyrase Multilocus sequence typing Fluoroquinolones Multilocus Sequence Typing Plasmids medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 69:2076-2080 |
| ISSN: | 1460-2091 0305-7453 |
| Popis: | OBJECTIVES To characterize the mechanisms implicated in fluoroquinolone (FQ) and expanded-spectrum cephalosporin (ESC) resistance in three clinical and seven faecal multidrug-resistant (MDR; resistant to at least three antimicrobial classes) Escherichia coli isolates from a dog with atopic dermatitis, also suffering from recurrent otitis, that had already been exposed to prolonged antimicrobial treatment and colonized for a long period. METHODS MICs of FQs, ESCs and other antimicrobials were determined by the broth microdilution method. Phenotypic tests (efflux pump inhibition and combination disc tests) and isoelectric focusing were combined with genotypic analyses [PCRs, sequencing, conjugation, S1 nuclease PFGE, PCR-based replicon typing, plasmid multilocus sequence typing (pMLST) and PCR mapping] to characterize the molecular basis of FQ and ESC resistance. Isolates were further characterized by MLST and PFGE. RESULTS Three otitis and five faecal isolates with enrofloxacin MICs of 32 to >128 mg/L displayed the GyrA:S83L+D87N/ParC:E62K/ParE:G545D pattern harbouring novel ParC and ParE substitutions, whereas the two remaining faecal isolates were susceptible or borderline resistant single-step mutants (GyrA:S83L pattern) and carried qnrS1. Efflux pump overexpression also contributed to FQ resistance and the MDR phenotype. The three otitis and five faecal isolates also exhibited cefoxitin/ceftazidime MICs of 32-64 mg/L and harboured blaCMY-2, adjusted to ISEcp1, on an IncI1/ST65 conjugative plasmid, previously described in Salmonella Heidelberg from poultry. Interestingly, all isolates shared an identical MLST type (ST212), with the otitis isolates showing indistinguishable patterns with the high-level resistant faecal E. coli isolates. CONCLUSIONS The long-term maintenance of FQ- and ESC-resistant clones harbouring topoisomerase mutations and a blaCMY-2-IncI1/ST65 plasmid in canine commensal flora after prolonged antimicrobial use may contribute to the dissemination of multidrug resistance. |
| Databáze: | OpenAIRE |
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