Uncoupling protein-3 mRNA up-regulation in C2C12 myotubes after etomoxir treatment
| Autor: | Manuel Vázquez, Rosa M. Sánchez, Marta Alegret, Àgatha Cabrero, Juan C. Laguna, Tomás Adzet |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Ceramide
Peroxisome proliferator-activated receptor Oxidative phosphorylation Biology Ceramides Ion Channels Cell Line Mitochondrial Proteins Mice chemistry.chemical_compound Sphingosine Animals Uncoupling Protein 3 Uncoupling protein RNA Messenger Enzyme Inhibitors Inner mitochondrial membrane Receptor Molecular Biology chemistry.chemical_classification Carnitine O-Palmitoyltransferase Reverse Transcriptase Polymerase Chain Reaction Cell Biology Molecular biology Up-Regulation Cell biology chemistry Epoxy Compounds Carnitine palmitoyltransferase I Carrier Proteins Etomoxir |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1532:195-202 |
| ISSN: | 1388-1981 |
| Popis: | Uncoupling proteins (UCPs) are mitochondrial membrane proton transporters that uncouple respiration from oxidative phosphorylation by dissipating the proton gradient across the membrane. Treatment of C2C12 myotubes for 24 h with 40 microM etomoxir, an irreversible inhibitor of carnitine palmitoyltransferase I (CPT-I), up-regulated uncoupling protein 3 (UCP-3) mRNA levels (2-fold induction), whereas UCP-2 mRNA levels were not modified. Etomoxir treatment also caused a 2.5-fold induction in M-CPT-I (muscle-type CPT-I) mRNA levels. In contrast, other well-known peroxisome proliferator-activated receptor alpha (PPAR alpha) target genes, such as acyl-CoA oxidase and medium-chain acyl-CoA dehydrogenase, were not affected, suggesting that this transcription factor was not involved in the effects of etomoxir. Since it has been reported that CPT-I inhibition by etomoxir leads to a further increase in ceramide synthesis, we test the possibility that ceramides were involved in the changes reported. Similarly to etomoxir, addition of 20 microM C(2)-ceramide to C2C12 myotubes for 3, 6 and 9 h resulted in increased UCP-3 and M-CPT-I mRNA levels. These results indicate that the effects on UCP-3 mRNA levels could be mediated by increased ceramide synthesis. |
| Databáze: | OpenAIRE |
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