Evaluation of double locus (clfB and spa) sequence typing for studying molecular epidemiology of methicillin-resistant Staphylococcus aureus in Taiwan
| Autor: | Chi Yuan Li, Cheng Mao Ho, Hui Chen Chen, Chen Cheng Huang, Ni Tien, Jang Jih Lu, Mao Cheng Ge, Hsiu Mei Fan |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Methicillin-Resistant Staphylococcus aureus Genotype 030106 microbiology Taiwan lcsh:QR1-502 Biology medicine.disease_cause lcsh:Microbiology Microbiology 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology(all) medicine Pulsed-field gel electrophoresis Immunology and Allergy Humans 030212 general & internal medicine Typing Adhesins Bacterial Genetics Antigens Bacterial Molecular Epidemiology General Immunology and Microbiology Molecular epidemiology Base Sequence SCCmec General Medicine Staphylococcal Infections biochemical phenomena metabolism and nutrition bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Electrophoresis Gel Pulsed-Field Infectious Diseases Staphylococcus aureus Staphylococcus Multilocus Sequence Typing double locus sequence typing |
| Zdroj: | Journal of Microbiology, Immunology and Infection, Vol 50, Iss 5, Pp 604-612 (2017) |
| ISSN: | 1684-1182 |
| DOI: | 10.1016/j.jmii.2015.10.002 |
| Popis: | Background Pulsed-field gel electrophoresis (PFGE) is the "gold standard" for epidemiological investigation of methicillin-resistant Staphylococcus aureus (MRSA), but several DNA sequence-based methods have been developed in MRSA typing because of the unambiguous results. Methods Ninety-one MRSA isolates were collected from the blood cultures of different patients from July 2008 to December 2008 in central Taiwan. The molecular characteristics of each isolate, including double locus sequence typing (DLST; spa and clfB typing), Staphylococcus cassette chromosome mec (SCCmec), and PFGE were determined for comparison. Results Five major clfB types (types A–E), 18 spa types, 33 DLST genotypes, five SCCmec types, 17 pulsotypes have been observed. Three major DLST genotypes (A1-t002, C0-t037, and B1-t437) and two major pulsotypes (6 and 8) were identified. Most clfB type A isolates (97.1%) were SCCmec type II and all clfB type C isolates (100%) were SCCmec type III. Most clfB type B isolates (88.9%) were SCCmec type IV (59.3%) and V T (29.6%). All (100%) clfB subtypes A1, A2, and C isolates and 70.4% of clfB type B isolates belonged to healthcare-associated-MRSA. The average congruence was 57.7% between DLST and PFGE, and 96.6% between clfB and SCCmec type. The index of discrimination of SCCmec, clfB , spa , PFGE, and DLST was 0.72, 0.79, 0.80, 0.81, and 0.87, respectively. Conclusion ClfB type has high congruence with SCCmec type. The DLST method in this study yielded a higher discriminatory power than PFGE in local investigation of molecular epidemiology of MRSA and a promising alternative to PFGE. |
| Databáze: | OpenAIRE |
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