Protective effects of Brussels sprouts towards B[a]P-induced DNA damage: a model study with the single-cell gel electrophoresis (SCGE)/Hep G2 assay
| Autor: | Fekadu Kassie, Ruud Verkerk, C. Freywald, Siegfried Knasmüller, Brenda Laky, G. Scharf, Maria Uhl, R. Gminski, Volker Mersch-Sundermann |
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| Rok vydání: | 2002 |
| Předmět: |
vegetables
extracts Carcinoma Hepatocellular DNA damage fruits Brassica in-vitro Toxicology chemistry.chemical_compound food Isothiocyanates Benzo(a)pyrene Cytochrome P-450 CYP1A1 Tumor Cells Cultured Animals Humans glutathione VLAG Glutathione Transferase Electrophoresis Agar Gel Dose-Response Relationship Drug Benzyl isothiocyanate Mutagenicity Tests Plant Extracts Leerstoelgroep Productontwerpen en kwaliteitskunde Liver Neoplasms Drug Synergism General Medicine Product Design and Quality Management Group Allyl isothiocyanate food.food Comet assay Hep G2 Enzyme Activation Biochemistry Sinigrin chemistry Glucosinolate BRUSSELS SPROUT benzyl isothiocyanate metabolizing enzymes allyl Food Science DNA Damage |
| Zdroj: | Food and Chemical Toxicology, 40(8), 1077-1083 Food and Chemical Toxicology 40 (2002) 8 |
| ISSN: | 0278-6915 |
| Popis: | The aim of this study was to investigate the chemoprotective effects of Brussels sprouts juice towards benzo[a]pyrene (B(a)P)-induced DNA damage in the single-cell gel electrophoresis (SCGE)/Hep G2 test system. This assay combines the advantages of the SCGE assay with that of the use of human-derived cells possessing inducible phase I and phase 11 enzymes. Co-treatment of Hep G2 cells with small amounts of Brussels sprouts juice (0.25-2.0 mul/ml) and B(a)P reduced the genotoxic effect of the latter in a dose-dependent manner. Contrary to the results with the crude juice, unexpected synergistic effects were observed with allyl isothiocyanate (AITC, 1.0-6.0 muM), a breakdown product of sinigrin, which is the most abundant glucosinolate in Brussels sprouts. Although these concentrations of AITC did not cause DNA damage per se, at higher concentrations (greater than or equal to 25 muM), the compound caused a pronounced dose-dependent DNA damage by itself. Mechanistic studies showed that Brussels sprouts juice causes induction of activities of ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST) at dose levels which were protective towards B(a)P. In combined treatment experiments with (+/-)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE, 5.0 muM), the main genotoxic metabolite of B(a)P, and Brussels sprouts juice, only weak protection was found indicating that the mechanism of chemoprotection of Brussels sprouts is not mediated through inactivation of this metabolite. In conclusion, our findings show that Brussels sprouts are highly protective against B(a)P-induced DNA damage in human-derived cells. (C) 2002 Elsevier Science Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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