A Microscale Technique for Gas Chromatography-Mass Spectrometry Measurements of Picogram Amounts of Indole-3-Acetic Acid in Plant Tissues
| Autor: | Göran Sandberg, Björn Sundberg, Staffan Eklöf, Thomas Moritz, A. Edlund |
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| Rok vydání: | 1995 |
| Předmět: |
Chromatography
biology Physiology Chemistry Nicotiana tabacum fungi food and beverages Plant Science Mass spectrometry biology.organism_classification chemistry.chemical_compound Genetics heterocyclic compounds Gas chromatography Gas chromatography–mass spectrometry Selectivity Indole-3-acetic acid Quantitative analysis (chemistry) Microscale chemistry Research Article |
| Zdroj: | Plant Physiology. 108:1043-1047 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.108.3.1043 |
| Popis: | A microscale technique has been developed for routine quantifications of picogram amounts of indole-3-acetic acid (IAA) in plant tissues by combined gas chromatography-mass spectrometry. Low- and high-resolution selected-ion-monitoring and selected-reaction-monitoring mass spectrometry techniques were compared for selectivity and precision. The best selectivity was obtained with selected-reaction-monitoring analysis, and 1-mg samples containing 500 fg of IAA could be analyzed accurately with this method. This technique was used to investigate the IAA distribution pattern along the longitudinal axis of tobacco (Nicotiana tabacum [L.]) leaves. In young, developing leaves an increase of endogenous IAA from the leaf tip to the base of the leaf was observed, whereas the level of IAA was uniform along this axis in mature leaves. |
| Databáze: | OpenAIRE |
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