Weight increase and overweight are associated with DNA oxidative damage in skeletal muscle
| Autor: | Laura Leiva, Daniela Olivares, Gladys Barrera, Mireya Fernández, María-Pía de la Maza, Walter Sierralta, Vivien Gattas, Daniel Bunout, Sandra Hirsch |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Aging medicine.medical_specialty Inflammation Overweight Weight Gain Critical Care and Intensive Care Medicine medicine.disease_cause Pathogenesis Internal medicine medicine Humans Obesity Muscle Skeletal Aged Aged 80 and over Aldehydes Nutrition and Dietetics Tumor Necrosis Factor-alpha business.industry Weight change Deoxyguanosine Skeletal muscle Middle Aged Anthropometry Immunohistochemistry Endocrinology medicine.anatomical_structure 8-Hydroxy-2'-Deoxyguanosine Case-Control Studies medicine.symptom business Oxidative stress DNA Damage |
| Zdroj: | CLINICAL NUTRITION Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 0261-5614 |
| DOI: | 10.1016/j.clnu.2006.02.008 |
| Popis: | Summary Background and aims Weight maintenance within normal standards is recommended for prevention of conditions associated with oxidative injury. To compare oxidative damage in a post mitotic tissue, between adults differing in long-term energy balance. Methods During hernia surgery, a sample of skeletal muscle was obtained in 17 non-obese adults. Subjects were divided into two groups according to their self-reported weight change: weight maintainers (WM) reported 5 kg increment. Muscle immunohistochemistry for 8-hydroxy-deoxyguanosine (8OHdG), 4-Hydroxy-2-nonenal (4HNE), and TNF-α, as markers of oxidative injury and inflammation, were performed. As known positive controls for oxidative injury, we included 10 elderly subjects (66–101 yr). Anthropometric measures and blood samples for clinical laboratory and serum cytokines (TNF-α and IL-6) were obtained. Results 8OHdG was higher in WG compared with WM (149.1±16.2 versus 117.8±29.5, P = 0.03 ), and was associated with anthropometric indicators of fat accumulation. 4HNE was similar in WG compared with WM (10.9±7.6 versus 9.8±6.3) but noticeably higher in elderly subjects (21.5±15.3, P = 0.059 ). TNF-α protein in WG was higher compared with WM (114.0±41.7 versus 70.1±23.3, P = 0.025 ), and was associated with weight increase. Conclusions Moderate self-reported weight increase, and body fat accumulation, suggesting long-term positive energy balance is associated with muscle DNA oxidative injury and inflammation. |
| Databáze: | OpenAIRE |
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