Improved stability of phycobiliprotein within liposome stabilized by polyethylene glycol adsorbed cellulose nanocrystals
| Autor: | Carl P. Tripp, SuriyaPrakaash Lakshmibalasubramaniam, Balunkeswar Nayak, Avinash Singh Patel, Abhijit Kar, Praveen Kumar Sappati |
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| Rok vydání: | 2020 |
| Předmět: |
Chemical Phenomena
02 engineering and technology Polyethylene glycol Phycobiliproteins Biochemistry Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound Adsorption Structural Biology Spectroscopy Fourier Transform Infrared Particle Size Cellulose Molecular Biology 030304 developmental biology 0303 health sciences Liposome biology Protein Stability Phycobiliprotein Temperature General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology biology.organism_classification Cellulose nanocrystals chemistry Palmaria palmata Liposomes Nanoparticles 0210 nano-technology Nuclear chemistry |
| Zdroj: | International journal of biological macromolecules. 163 |
| ISSN: | 1879-0003 |
| Popis: | This study ascertained the stability of phycobiliprotein (PBP), a bioactive protein from Dulse (Palmaria palmata) loaded within liposomes and stabilized with polyethylene glycol (2000 and 4000 g/mol) and desulfated CNCs (DCs) containing adsorbed polyethylene glycol (DCs-2000 and DCs-4000). The effect of pH, temperature and illumination on the stability of PBP was investigated. Results showed that the temperature had the most significant (p 0.05) effect on the fluorescence intensity of the PBP, accounting for up to 70% loss of the fluorescence intensity for PBP loaded liposome (PL), PL stabilized with PEG-2000 (PLP-2000) and PEG 4000 (PLP-4000) and PL stabilized with desulfated CNCs (DCs), however, 60% for the PL stabilized with PEG 2000 and PEG 4000 adsorbed CNCs (PLDCs-2000 and PLDCs-4000) at 60 °C compared to those stabilized at 4 °C. A further increase in temperature to 80 °C led to a complete loss of fluorescence. Operating at the extreme pH's of 1.0 and 11.0 resulted in a loss of 90% and 30% fluorescence intensity, respectively for PBP in solution, whereas, 20% and 2% loss was observed for PBP incorporated inside the liposomes. Regarding the effect of illumination, PLDCs-2000 and PLDCs-4000 were the most stable, retaining the fluorescence intensity of PBP up to 70% after 72 h of exposure. This is compared to 85% loss of fluorescence for PBP in solution. Furthermore, at pH of 1.0, there was an increase in average particle size for the PLDCs-2000 and PLDCs-4000 from 189 ± 3206 ± 2 nm to 6464 ± 2116698 ± 317 nm and a charge reversal in the zeta potential from -36 ± 1-34 ± 2 to +16 ± 3+14 ± 1. Confocal and optical microscopic images confirmed the coalescence of PBP loaded liposome and agglomeration PLDCs-2000 and PLDCs-4000 under acidic pH (3.0). In contrast, changes in temperature from 4 °C to 100 °C and illumination as a function of time up to 72 h resulted in no change in liposome size and zeta potential. |
| Databáze: | OpenAIRE |
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