Determination of subnanogram concentrations of fentanyl in plasma by gas chromatography—mass spectrometry: Comparison with standard radioimmunoassay
| Autor: | B, Fryirs, A, Woodhouse, J L, Huang, M, Dawson, L E, Mather, B, Fryirsa |
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| Rok vydání: | 1997 |
| Předmět: |
Detection limit
Chromatography Chemistry Radioimmunoassay General Chemistry Mass spectrometry Sensitivity and Specificity Gas Chromatography-Mass Spectrometry Analgesics Opioid Fentanyl Injections Intravenous Blood plasma Humans Sample preparation Reagent Kits Diagnostic Gas chromatography Gas chromatography–mass spectrometry Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 688:79-85 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(97)88058-9 |
| Popis: | A method was devised to determine fentanyl plasma concentrations by GC-MS using selected-ion monitoring (SIM) with sufentanil as internal standard. This was compared with a commonly used commercial radioimmunoassay (RIA). Sample preparation for GC-MS involved basification of plasma then extraction using n-butyl chloride followed by concentration to dryness and reconstitution in toluene for chromatography. Using 1-ml plasma samples, the estimated limit of detection of fentanyl was 20 pg/ml. Blood samples for pharmacokinetic studies were split and assayed by GC-MS and RIA which had a limit of detection of 200 pg/ml. Pearson's r (r - 0.80, p0.0001) indicated the methods were highly correlated at all plasma concentrations. Owing to the greater sensitivity of the method, GC-MS is recommended over RIA for subnanogram determination of fentanyl in plasma. |
| Databáze: | OpenAIRE |
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