Plant nitrogen supply affects the Botrytis cinerea infection process and modulates known and novel virulence factors

Autor: Elise Loisel, Amelie Devallee, Baptiste Vancostenoble, Antoine Daviere, Nathalie Poussereau, Dominique Expert, Kévin Floch, Shamsuddin Mia Koka, Marie-Christine Soulié, Veronique Brunaud, Mathilde Fagard, Deborah Barbe, Ludivine Soubigou-Taconnat
Přispěvatelé: Sorbonne Université - UFR Sciences de la vie (UFR 927 ), Sorbonne Université (SU), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microbiologie, adaptation et pathogénie (MAP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Génomique fonctionnelle des Champignons Phytopathogènes (GFCP), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), INRA-BAP (Nitropath BAP2014_63), ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Molecular Plant Pathology
Molecular Plant Pathology, Wiley, 2020, 21, pp.1436-1450. ⟨10.1111/mpp.12984⟩
Molecular Plant Pathology, 2020, 21, pp.1436-1450. ⟨10.1111/mpp.12984⟩
ISSN: 1364-3703
1464-6722
DOI: 10.1111/mpp.12984⟩
Popis: Plant nitrogen (N) fertilization is known to affect disease; however, the underlying mechanisms remain mostly unknown. We investigated the impact of N supply on the Arabidopsis thaliana–Botrytis cinerea interaction. A. thaliana plants grown in low nitrate were more tolerant to all wild‐type B. cinerea strains tested. We determined leaf nitrate concentrations and showed that they had a limited impact on B. cinerea growth in vitro. For the first time, we performed a dual RNA‐Seq of infected leaves of plants grown with different nitrate concentrations. Transcriptome analysis showed that plant and fungal transcriptomes were marginally affected by plant nitrate supply. Indeed, only a limited set of plant (182) and fungal (22) genes displayed expression profiles altered by nitrate supply. The expression of selected genes was confirmed by quantitative reverse transcription PCR at 6 hr postinfection (hpi) and analysed at a later time point (24 hpi). We selected three of the 22 B. cinerea genes identified for further analysis. B. cinerea mutants affected in these genes were less aggressive than the wild‐type strain. We also showed that plants grown in ammonium were more tolerant to B. cinerea. Furthermore, expression of the selected B. cinerea genes in planta was altered when plants were grown with ammonium instead of nitrate, demonstrating an impact of the nature of N supplied to plants on the interaction. Identification of B. cinerea genes expressed differentially in planta according to plant N supply unveils two novel virulence functions required for full virulence in A. thaliana: a secondary metabolite (SM) and an acidic protease (AP).
Nitrogen affects the Arabidopsis–Botrytis interaction. We identified 182 Arabidopsis and 22 Botrytis genes affected by nitrate supply. Botrytis cinerea mutants affected in three genes were less aggressive than the wild‐type.
Databáze: OpenAIRE
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