Description of an automatic copulation induction system used to establish a free-mating laboratory colony of Nyssorhynchus deaneorum from Brazil
| Autor: | Fábio Resadore, Raphael Brum Castro, Luiz Hidelbrando Pereira-da-Silva, Alessandra da Silva Bastos, Jansen Fernandes Medeiros, Maisa da Silva Araujo, Alice Oliveira Andrade, Najara Akira Costa dos Santos |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Oviposition RC955-962 030231 tropical medicine Plasmodium vivax Plasmodium falciparum Zoology Mosquito Vectors Microbiology Induction system 03 medical and health sciences 0302 clinical medicine malaria vector Arctic medicine. Tropical medicine parasitic diseases Anopheles Copulation Nyssorhynchus Animals copulation induction Mating Malaria vector biology colony establishment F1 generation biology.organism_classification QR1-502 Nyssorhynchus deaneorum Insect Vectors Malaria Pupa Female Original Article Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz, Volume: 115, Article number: e200070, Published: 08 JUL 2020 Memórias do Instituto Oswaldo Cruz., Vol 115 (2020) |
| ISSN: | 1678-8060 |
| Popis: | BACKGROUND Nyssorhynchus deaneorum is a potential malaria vector because it has been shown to be competent to transmit Plasmodium vivax and Plasmodium falciparum, and because it exhibits antropophilic and endophilic behaviors in some regions of the Amazon. This profile makes Ny. deaneorum a useful mosquito for experiments that model Plasmodium-vector interactions in the Amazon. OBJECTIVE Herein we describe how a free-mating colony of Ny. deaneorum has been established using an automated light stimulation system. METHODS Mosquitoes were captured in São Francisco do Guaporé, Rondônia. The F1 generation was reared until adult emergence at which point copulation was induced using an automatic copulation induction system (ACIS). FINDINGS After four generations, natural mating and oviposition began to occur without light stimulation. The number of pupae and adult mosquitoes increased from the F5 to F10 generations. The new Ny. deaneorum colony exhibited susceptibility to P. vivax. MAIN CONCLUSIONS Automated light stimulation is an effective method for establishing an Ny. deaneorum colony under laboratory conditions as it produces enough adults to create a stenogamic colony. The establishment of a stable, P. vivax-susceptible colony of Ny. deaneorum makes it possible to model parasite-vector interactions and to test novel drug therapies that target parasite development in mosquitoes. |
| Databáze: | OpenAIRE |
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