Pro-inflammatory cytokine profile is present in the serum of Mexican patients with different stages of diabetic retinopathy secondary to type 2 diabetes
| Autor: | Jonathan Uriel Quevedo-Martínez, Diana Venegas, Victor Manuel Bautista-de Lucio, Joanna Jimenez, Osvaldo Garcia, Yonathan Garfias |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment Type 2 diabetes Gastroenterology Retina Proinflammatory cytokine immunology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Blood serum Internal medicine Diabetes mellitus medicine Creatinine business.industry Interleukin Diabetic retinopathy RE1-994 medicine.disease diagnostic tests/investigation Ophthalmology 030104 developmental biology Cytokine chemistry inflammation 030221 ophthalmology & optometry business |
| Zdroj: | BMJ Open Ophthalmology, Vol 6, Iss 1 (2021) BMJ Open Ophthalmology |
| ISSN: | 2397-3269 |
| Popis: | AimIt’s been reported that pro-inflammatory cytokines are elevated in patients with diabetic retinopathy (DR); this may contribute to the pathophysiology of the disease. The aim of this study is to measure the concentration of various inflammatory cytokines from the main CD4+ T helper inflammatory responses in blood serum from Mexican patients with DR in different stages using cytometric bead array (CBA) technology and correlate them with the presence and severity of DR in order to find possible DR biomarkers that serve as diagnostic or therapeutic predictors.Methods64 subjects were included in the study, 16 in the control group, 16 in the type 2 diabetes mellitus no DR (NDR) group, 16 in the non-proliferative DR (NPDR) group and 16 in the proliferative DR (PDR) group. Cytokine concentrations of interleukin (IL) 1ß, IL‐2, IL‐4, IL‐6, IL‐8, IL‐10, IL‐12, IL‐17A, tumour necrosis factor alpha (TNFα) and interferon-gamma in serum samples were measured using Human Inflammatory and TH1/TH2/TH17 CBA Kit.ResultsIL-6, IL-12, IL-17a and TNFα were significantly higher in the patients with DR compared with the control group. The PDR group showed a slightly lower concentration of serum cytokines IL-6, IL-12 and IL-17a. TNFα showed a higher concentration compared with healthy controls, NDR and NPDR subjects. We also found a positive statistical correlation between the presence and severity of DR with the clinical parameters haemoglobin A1c, body mass index and serum creatinine and the concentration of serum cytokines IL-6 and TNFα.ConclusionOur findings suggest that patients with diabetes and DR have a stronger chronic inflammatory profile compared with non-diabetic subjects. |
| Databáze: | OpenAIRE |
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