IL-1ra and IL-1 production in human oral mucosal epithelial cells in culture: differential modulation by TGF-β1 and IL-4
| Autor: | B. Kherratia, B. Sauvezie, S. Perrier, J. L. Kemeny, M. Baudet-Pommel, C. Deschaumes, S. Ughetto |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Adolescent Sialoglycoproteins medicine.medical_treatment Immunology Biology Immune tolerance Transforming Growth Factor beta1 Immune system Adjuvants Immunologic Western blot Transforming Growth Factor beta medicine Humans Protein Isoforms Immunology and Allergy Immunity Mucosal Involucrin Cells Cultured Interleukin 4 medicine.diagnostic_test Monocyte Mouth Mucosa Epithelial Cells Interleukin 1 Receptor Antagonist Protein medicine.anatomical_structure Cytokine Interleukin 1 receptor antagonist Animal Studies Interleukin-4 Interleukin-1 |
| Zdroj: | Clinical and Experimental Immunology. 127:53-59 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | SummaryRegulatory cytokines mediate the participation of oral mucosal epithelial cells (OMEC) in local immune responses. The aim of this study was to characterize the isoforms of IL-1 receptor antagonist (IL-1ra) in cultured human primary OMECs and to compare its production with that of IL-1 alpha (IL-1α) and IL-1 beta (IL-1β). Western blot analysis showed that IL-1ra was 22 kDa in size hence slightly smaller than monocyte IL-1ra (25 kDa). A minor form of 20 kDa was also found in unstimulated cell culture lysates. In culture supernatants, IL-1 bioactivity increased after IL-1ra neutralization, indicating that the baseline production of IL-1ra is biologically relevant. Immunohistochemistry showed a relation between IL-1ra and involucrin expressions, suggesting that intracytoplasmic IL-1ra may be involved in cell terminal differentiation. In unstimulated culture lysates, there was far more IL-1ra than IL-1α and IL-1β. TGF-β1 markedly increased the IL-1ra/IL-1β ratio from 93·6 : 1 to 300 : 1. IL-4, which is generally described as an anti-inflammatory cytokine, increased IL-1 but not IL-1ra production. TNF-α increased intracellular production of the three IL-1 members. IL-1ra levels were lower in supernatants than in lysates of cultured cells. Our results show that human OMECs constitutively produce significant amounts of a biologically active form of IL-1ra. TGF-β1 µp-regulation points to a positive amplification loop and IL-4 to a down-regulation loop, both including Th2 cells and OMECs. They may be important in oral tolerance and IgA production, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|