The Effect of Pesticides on the Tomato Bacterial Speck Disease Pathogen Pseudomonas Syringae pv. Tomato
| Autor: | Antonina Kalinichenko, Yuliia Kolomiiets, Liudmyla Butsenko, Lidiia Pasichnyk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microorganism
Mutant 010501 environmental sciences Biology 01 natural sciences lcsh:Technology Microbiology lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound morphological dissociation Pseudomonas syringae pv. tomato antibacterial activity Pseudomonas syringae General Materials Science Instrumentation Pathogen lcsh:QH301-705.5 030304 developmental biology 0105 earth and related environmental sciences Fluid Flow and Transfer Processes 0303 health sciences aluminium phosethyl lcsh:T Process Chemistry and Technology fungi General Engineering food and beverages pesticides deltamethrin Pesticide biology.organism_classification lcsh:QC1-999 Computer Science Applications Fungicide Deltamethrin chemistry lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 mutagenic action lcsh:Engineering (General). Civil engineering (General) Bacteria lcsh:Physics |
| Zdroj: | Applied Sciences, Vol 10, Iss 3263, p 3263 (2020) Applied Sciences Volume 10 Issue 9 |
| ISSN: | 2076-3417 |
| Popis: | A significant part of the used pesticides does not reach the target organisms and, while remaining in the agrophytocenosis, influences all living organisms in it. Having a toxic and often mutagenic effect, pesticides induce morphological and physiological changes in the cells of microorganisms and are the cause of phenotypic heterogeneity of their populations. However, the effect of pesticides on phytopathogenic bacteria as non-target microorganisms remains out of the field of view for most researchers. However, the use of pesticides can lead to expansion of the diversity of existing phytopathogens and, as a consequence, complications of identification of the pathogens, loss of resistance by plants varieties, and increased harm from diseases caused by them. This study is focused on the effect of pesticides used in tomato plantations on the causative agent of bacterial speck of this crop&mdash Pseudomonas syringae pv. tomato. The studies were carried out using the methods of classical microbiology. The mutagenic action of pesticides was recorded, taking into account the increase of the number of streptomycin resistance mutations in bacteria in the case of pesticide action. It is established that the fungicide aluminium phosethyl is characterised by a bacteriostatic effect on P. syringae pv. tomato. Deltamethrin insecticide does not affect the growth of P. syringae pv. tomato. However, there is an increase in the frequency of streptomycin resistance mutations in both studied strains of P. syringae pv. tomato after using deltamethrin. It is shown that the frequency of occurrence of R (rough colonies) forms of P. syringae pv. tomato IZ28 and IZ46 after using deltamethrin increased by 100 times when in comparison to the frequency of spontaneous morphological dissociation, or smooth-to-rough (S-R) mutation, of these bacteria. Therefore, aluminium phosethyl is characterised by moderate bacteriostatic action against P. syringae pv. tomato. Deltamethrin does not influence the growth of the pathogen of tomato speck but increases the frequency of formation of StrR mutants and R forms of phytopathogenic bacteria. |
| Databáze: | OpenAIRE |
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