Assessment of the genetic risks of a metallic alloy used in medical implants
| Autor: | Cristiano C. Gomes, Juliana Pereira Lyon, Fábio Vieira dos Santos, Leonardo Marmo Moreira, Vanessa J. S. V. Santos, Alfeu Saraiva Ramos, Cristina Pacheco Soares |
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| Rok vydání: | 2011 |
| Předmět: |
lcsh:QH426-470
DNA damage biomaterial technology industry and agriculture mutagenicity Biomaterial Titanium alloy Biology medicine.disease_cause lcsh:Genetics Mutagenesis Micronucleus test Genetics medicine cytotoxicity micronucleus MTT assay Ti-6Al-4V Viability assay Micronucleus Molecular Biology Genotoxicity Research Article Biomedical engineering |
| Zdroj: | Genetics and Molecular Biology Genetics and Molecular Biology, Volume: 34, Issue: 1, Pages: 116-121, Published: 07 JAN 2011 Genetics and Molecular Biology, Vol 34, Iss 1, Pp 116-121 (2011) Genetics and Molecular Biology v.34 n.1 2011 Sociedade Brasileira de Genética (SBG) instacron:SBG |
| ISSN: | 1678-4685 1415-4757 |
| DOI: | 10.1590/s1415-47572010005000118 |
| Popis: | The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial for orthopedic implants, but the toxicity of the chemical components of this alloy has become an issue of concern. In this work, we used the MTT assay and micronucleus assay to examine the cytotoxicity and genotoxicity, respectively, of an extract obtained from this alloy. The MTT assay indicated that the mitochondrial activity and cell viability of CHO-K1 cells were unaffected by exposure to the extract. However, the micronucleus assay revealed DNA damage and an increase in micronucleus frequency at all of the concentrations tested. These results show that ions released from Ti-6Al-4V alloy can cause DNA and nuclear damage and reinforce the importance of assessing the safety of metallic medical devices constructed from biomaterials. |
| Databáze: | OpenAIRE |
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