HMGA1-pseudogene7 transgenic mice develop B cell lymphomas
| Autor: | Sara Carmela Credendino, Eugenio Gaudio, Paolo Chieffi, Floriana Forzati, Davide De Biase, Orlando Paciello, Francesco Esposito, Marco De Martino, Giuseppe De Palma, Antonio Barbieri, Alfredo Fusco, Francesco Bertoni, Maurilio Ponzoni, Gabriella De Vita, Claudio Arra |
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| Přispěvatelé: | De Martino, M., De Biase, D., Forzati, F., Credendino, S. C., Palma, G., Barbieri, A., Arra, C., Paciello, O., Gaudio, E., Ponzoni, M., De Vita, G., Chieffi, P., Bertoni, F., Fusco, A., Esposito, F., Vita, G. D. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Genetically modified mouse
Lymphoma B-Cell Lymphoma Pseudogene Transgene lcsh:Medicine Mice Transgenic Biology Article Animals Flow Cytometry HMGA1a Protein Immunohistochemistry Lymphocytes Mice NIH 3T3 Cells Pseudogenes RNA-Seq Transgenic Cancer epigenetics microRNA medicine HMGA1-pseudogene7 transgenic B cell lymphomas lcsh:Science B-cell lymphoma B cell Multidisciplinary lcsh:R B-Cell Cancer Oncogenes medicine.disease Publisher Correction medicine.anatomical_structure Cancer research lcsh:Q |
| Zdroj: | Scientific reports (Nature Publishing Group) 10 (2020). doi:10.1038/s41598-020-62974-0 info:cnr-pdr/source/autori:De Martino M.; De Biase D.; Forzati F.; Credendino S.C.; Palma G.; Barbieri A.; Arra C.; Paciello O.; Gaudio E.; Ponzoni M.; Vita G.D.; Chieffi P.; Bertoni F.; Fusco A.; Esposito F./titolo:HMGA1-pseudogene7 transgenic mice develop B cell lymphomas/doi:10.1038%2Fs41598-020-62974-0/rivista:Scientific reports (Nature Publishing Group)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:10 Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
| DOI: | 10.1038/s41598-020-62974-0 |
| Popis: | We have recently identified and characterized two pseudogenes (HMGA1P6 and HMGA1P7) of the HMGA1 gene, which has a critical role in malignant cell transformation and cancer progression. HMGA1P6 and HMGAP17 act as microRNA decoy for HMGA1 and other cancer-related genes upregulating their protein levels. We have previously shown that they are upregulated in several human carcinomas, and their expression positively correlates with a poor prognosis and an advanced cancer stage. To evaluate in vivo oncogenic activity of HMGA1 pseudogenes, we have generated a HMGA1P7 transgenic mouse line overexpressing this pseudogene. By a mean age of 12 months, about 50% of the transgenic mice developed splenomegaly and accumulation of lymphoid cells in several body compartments. For these mice FACS and immunohistochemical analyses suggested the diagnosis of B-cell lymphoma that was further supported by clonality analyses and RNA expression profile of the pathological tissues of the HMGA1P7 transgenic tissues. Therefore, these results clearly demonstrate the oncogenic activity of HMGA1 pseudogenes in vivo. |
| Databáze: | OpenAIRE |
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