Development of a Microforce Sensor and Its Array Platform for Robotic Cell Microinjection Force Measurement
| Autor: | Lingyun Wang, Yunlei Zhou, Wenming Xi, Yuzi Lin, Yu Xie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0209 industrial biotechnology
Engineering Microinjections Cell Transplantation cell-holding device cellular force sensor force measurement microinjection micromanipulation PVDF film 02 engineering and technology Biosensing Techniques lcsh:Chemical technology Biochemistry Article Analytical Chemistry Cell Line Mice 020901 industrial engineering & automation Electronic engineering Animals lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Throughput (business) Injection force Microinjection Zebrafish business.industry technology industry and agriculture Linearity Robotics 021001 nanoscience & nanotechnology Piezoelectricity Atomic and Molecular Physics and Optics Scale dependent Robot Artificial intelligence Stress Mechanical 0210 nano-technology business |
| Zdroj: | Sensors (Basel, Switzerland) Sensors; Volume 16; Issue 4; Pages: 483 Sensors, Vol 16, Iss 4, p 483 (2016) |
| ISSN: | 1424-8220 |
| Popis: | Robot-assisted cell microinjection, which is precise and can enable a high throughput, is attracting interest from researchers. Conventional probe-type cell microforce sensors have some real-time injection force measurement limitations, which prevent their integration in a cell microinjection robot. In this paper, a novel supported-beam based cell micro-force sensor with a piezoelectric polyvinylidine fluoride film used as the sensing element is described, which was designed to solve the real-time force-sensing problem during a robotic microinjection manipulation, and theoretical mechanical and electrical models of the sensor function are derived. Furthermore, an array based cell-holding device with a trapezoidal microstructure is micro-fabricated, which serves to improve the force sensing speed and cell manipulation rates. Tests confirmed that the sensor showed good repeatability and a linearity of 1.82%. Finally, robot-assisted zebrafish embryo microinjection experiments were conducted. These results demonstrated the effectiveness of the sensor working with the robotic cell manipulation system. Moreover, the sensing structure, theoretical model, and fabrication method established in this study are not scale dependent. Smaller cells, e.g., mouse oocytes, could also be manipulated with this approach. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|