Changes in the Expression of Various Transporters as Influencing Factors of Resistance to Cisplatin

Autor: Shoji Fukushima, Megumi Yasuda, Shuichi Kishimoto
Rok vydání: 2017
Předmět:
0301 basic medicine
Carcinoma
Hepatocellular
Cell Survival
Cell
Population
Antineoplastic Agents
law.invention
Inhibitory Concentration 50
03 medical and health sciences
0302 clinical medicine
law
medicine
Humans
RNA
Messenger
education
Cation Transport Proteins
Polymerase chain reaction
Copper Transporter 1
Cisplatin
education.field_of_study
Dose-Response Relationship
Drug
Chemistry
Liver Neoplasms
Membrane Transport Proteins
Transporter
Hep G2 Cells
Molecular biology
Multidrug Resistance-Associated Protein 2
female genital diseases and pregnancy complications
Gene Expression Regulation
Neoplastic
Multiple drug resistance
030104 developmental biology
medicine.anatomical_structure
Drug Resistance
Neoplasm
Cell culture
030220 oncology & carcinogenesis
Multidrug Resistance-Associated Proteins
Intracellular
medicine.drug
Zdroj: Anticancer Research. 37
DOI: 10.21873/anticanres.11977
Popis: BACKGROUND Changes in the expression of transporters have been reported as factors in resistance to cisplatin (CDDP). This study was designed to clarify whether CDDP-resistant strains isolated from a cell line had the same characteristics, and whether these characteristics could be therapeutic targets. MATERIALS AND METHODS Intracellular platinum levels were determined by the inductively-coupled plasma method. mRNA expression levels were determined using the real-time polymerase chain reaction. RESULTS Some CDDP-resistant HepG2 cell lines exhibited changes in the expression of copper transporter 1, multidrug resistant protein (MRP)2, and/or MRP3, resulting in decreased intracellular platinum amounts, while others showed no change in platinum accumulation. Expression of these transporters was not necessarily maintained in a constant direction within the cell population isolated from the same origin. CONCLUSION These results suggest that the CDDP-resistant tumors caused by a decrease in intracellular platinum content consist of a heterogeneous cell population showing expression changes of several transporters.
Databáze: OpenAIRE
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