Decreased Excretion of Urinary Exosomal Aquaporin-2 in a Puromycin Aminonucleoside-Induced Nephrotic Syndrome Model

Autor: Ayaha Kaito, Naruki Fujimoto, Masahiro Ikeda, Ahmed Abdeen, Hiroko Sonoda, Sayaka Oshikawa-Hori
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Nephrotic Syndrome
medicine.medical_treatment
aquaporin-2
030232 urology & nephrology
Urine
urologic and male genital diseases
Exosomes
lcsh:Chemistry
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
Proteinuria
nephrotoxicity
General Medicine
Computer Science Applications
Aquaporin 2
medicine.symptom
Injections
Intraperitoneal
medicine.medical_specialty
puromycin aminonucleoside
Urinary system
Intraperitoneal injection
Down-Regulation
Catalysis
Article
Nephrotoxicity
Inorganic Chemistry
Excretion
03 medical and health sciences
Internal medicine
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
business.industry
urogenital system
Organic Chemistry
medicine.disease
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
business
Nephrotic syndrome
urinary exosomes
Biomarkers
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 21, Iss 4288, p 4288 (2020)
Volume 21
Issue 12
ISSN: 1422-0067
Popis: Urinary exosomes, small extracellular vesicles present in urine, are secreted from all types of renal epithelial cells. Aquaporin-2 (AQP2), a vasopressin-regulated water channel protein, is known to be selectively excreted into the urine through exosomes (UE-AQP2), and its renal expression is decreased in nephrotic syndrome. However, it is still unclear whether excretion of UE-AQP2 is altered in nephrotic syndrome. In this study, we examined the excretion of UE-AQP2 in an experimental rat model of nephrotic syndrome induced by the administration of puromycin aminonucleoside (PAN). Rats were assigned to two groups: a control group administered saline and a PAN group given a single intraperitoneal injection of PAN (125 mg/kg) at day 0. The experiment was continued for 8 days, and samples of urine, blood, and tissue were collected on days 2, 5, and 8. The blood and urine parameters revealed that PAN induced nephrotic syndrome on days 5 and 8, and decreases in the excretion of UE-AQP2 were detected on days 2 through 8 in the PAN group. Immunohistochemistry showed that the renal expression of AQP2 was decreased on days 5 and 8. The release of exosomal marker proteins into the urine through UEs was decreased on day 5 and increased on day 8. These data suggest that UE-AQP2 is decreased in PAN-induced nephrotic syndrome and that this reflects its renal expression in the marked proteinuria phase after PAN treatment.
Databáze: OpenAIRE
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