An endoplasmic reticulum targeting green fluorescent protein chromophore-based probe for the detection of viscosity

Autor:	Xiaoqin Wei, Yiming Zhu, Xiang Yu, Lei Cai, Nanan Ruan, Luling Wu, Nengqin Jia, Tony D. James, Chusen Huang
Rok vydání:	2022
Předmět:	Viscosity Green Fluorescent Proteins Autophagy Materials Chemistry Metals and Alloys Ceramics and Composites General Chemistry Endoplasmic Reticulum Endoplasmic Reticulum Stress Catalysis Fluorescent Dyes Surfaces Coatings and Films Electronic Optical and Magnetic Materials
Zdroj:	Chemical Communications. 58:10727-10730
ISSN:	1364-548X 1359-7345
Popis:	The occurrence of endoplasmic reticulum (ER) stress is the main cause of a variety of biological processes that are closely related to numerous diseases. The homeostasis of the ER microenvironment can be disrupted under ER stress. In this research, by linking a pentafluorophenyl to the green fluorescent protein chromophore, we have developed a new ER-targeting fluorescent probe (GE-Y) for measuring changes of intracellular ER viscosity caused by ER stress. Importantly, an increase in ER viscosity was observed using GE-Y in cells undergoing autophagy. As such, our research provides an ideal tool for studying ER stress and autophagy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::324d1870bde64e23025b5c71d4f1bc8d https://doi.org/10.1039/d2cc00118g Zobrazit plný text záznamu