Identification of Novel Equine (Equus caballus) Tendon Markers Using RNA Sequencing
| Autor: | Peter J. Richards, Felix Theiss, Jan M. Kuemmerle, Ali Mirsaidi, Sonja Hemmi, Fabienne A. Weber, Paolo Cinelli, Michal J. Okoniewski |
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| Přispěvatelé: | University of Zurich, Richards, Peter J |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
eyes absent homolog 2 2716 Genetics (clinical) Cell type tendon G-protein regulated inducer of neurite outgrowth 3 lcsh:QH426-470 040301 veterinary sciences 610 Medicine & health Matrix (biology) Biology Article horse transcriptome Eyes absent homolog 2 0403 veterinary science Transcriptome 03 medical and health sciences 1311 Genetics Tissue engineering Gene expression Genetics medicine 10239 Institute of Laboratory Animal Science Gene Genetics (clinical) 11077 Center for Applied Biotechnology and Molecular Medicine Cartilage 04 agricultural and veterinary sciences musculoskeletal system Molecular biology Tendon 10021 Department of Trauma Surgery lcsh:Genetics 030104 developmental biology medicine.anatomical_structure 10090 Equine Department |
| Zdroj: | Genes, Vol 7, Iss 11, p 97 (2016) Genes; Volume 7; Issue 11; Pages: 97 Genes Genes, 7 (11) |
| ISSN: | 2073-4425 |
| Popis: | Although several tendon-selective genes exist, they are also expressed in other musculoskeletal tissues. As cell and tissue engineering is reliant on specific molecular markers to discriminate between cell types, tendon-specific genes need to be identified. In order to accomplish this, we have used RNA sequencing (RNA-seq) to compare gene expression between tendon, bone, cartilage and ligament from horses. We identified several tendon-selective gene markers, and established eyes absent homolog 2 (EYA2) and a G-protein regulated inducer of neurite outgrowth 3 (GPRIN3) as specific tendon markers using RT-qPCR. Equine tendon cells cultured as three-dimensional spheroids expressed significantly greater levels of EYA2 than GPRIN3, and stained positively for EYA2 using immunohistochemistry. EYA2 was also found in fibroblast-like cells within the tendon tissue matrix and in cells localized to the vascular endothelium. In summary, we have identified EYA2 and GPRIN3 as specific molecular markers of equine tendon as compared to bone, cartilage and ligament, and provide evidence for the use of EYA2 as an additional marker for tendon cells in vitro. Genes, 7 (11) ISSN:2073-4425 |
| Databáze: | OpenAIRE |
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