Functional and Morphological Development of Lymphoid Tissues and Immune Regulatory and Effector Function in Rhesus Monkeys: Cytokine-Secreting Cells, Immunoglobulin-Secreting Cells, and CD5+B-1 Cells Appear Early in Fetal Development
| Autor: | Tracy Rourke, Marta L. Marthas, Norbert Makori, Andrew G Hendrickx, Christopher J. Miller, Michael B. McChesney, Alice F. Tarantal, Fabien X. Lü |
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| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Lymphocyte Clinical Biochemistry Immunology Antigen-Presenting Cells Immunoglobulins CD5 Antigens Immunophenotyping Embryonic and Fetal Development Interleukin 21 Fetus Immune system hemic and lymphatic diseases Cellular Immunology medicine Animals Immunology and Allergy Antigen-presenting cell CD40 biology Macrophages Dendritic Cells Immunohistochemistry Macaca mulatta Lymphocyte Subsets Intestines B-1 cell medicine.anatomical_structure Immune System biology.protein Cytokines Lymph Nodes Antibody CD5 Spleen |
| Zdroj: | Clinical and Vaccine Immunology. 10:140-153 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cdli.10.1.140-153.2003 |
| Popis: | Little is known regarding the timing of immune ontogeny and effector function in fetal humans and nonhuman primates. We studied the organization of lymphocyte and antigen-presenting cell populations in developing lymphoid tissues of rhesus monkey fetuses during the second and third trimesters (65 to 145 days of gestation; term = 165 days). Immunoglobulin-secreting and cytokine-secreting cells were detected at day 80. The thymus, spleen, lymph nodes, and intestinal mucosa were examined for cells expressing CD3, CD5, CD20, CD68, p55, and HLA-DR. In the spleens of 65-day-old fetuses (early second trimester), the overwhelming majority of total lymphocytes were CD5+CD20+B-1 cells. The remaining lymphocytes were CD3+T cells. By day 80, splenic B and T cells were equal in number. Intraepithelial CD3+CD5−T cells and lamina propria CD20+CD5+B cells were present in the intestines of 65-day-old fetuses. By day 80, numerous CD20+CD5+B cells were present in the jejunums and colons and early lymphocyte aggregate formation was evident. The spleens of 80- to 145-day-old fetuses contained immunoglobulin M (IgM)-secreting cells, while IgA-, IgG-, interleukin-6-, and gamma interferon-secreting cells were numerous in the spleens and colons. Thus, by the second trimester, the lymphoid tissues of the rhesus monkey fetus have a complete repertoire of properly organized antigen-presenting cells, T cells, and B cells. |
| Databáze: | OpenAIRE |
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