Antisense-mediated repression of SAGA-dependent genes involves the HIR histone chaperone

Autor: Julien Soudet, Nissrine Beyrouthy, Anna Marta Pastucha, Andrea Maffioletti, Dario Menéndez, Zahra Bakir, Françoise Stutz
Rok vydání: 2022
Předmět:
Zdroj: Nucleic Acids Research. 50:4515-4528
ISSN: 1362-4962
0305-1048
DOI: 10.1093/nar/gkac264
Popis: Eukaryotic genomes are pervasively transcribed by RNA polymerase II (RNAPII), and transcription of long non-coding RNAs often overlaps with coding gene promoters. This might lead to coding gene repression in a process named Transcription Interference (TI). In Saccharomyces cerevisiae, TI is mainly driven by antisense non-coding transcription and occurs through re-shaping of promoter Nucleosome-Depleted Regions (NDRs). In this study, we developed a genetic screen to identify new players involved in Antisense-Mediated Transcription Interference (AMTI). Among the candidates, we found the HIR histone chaperone complex known to be involved in de novo histone deposition. Using genome-wide approaches, we reveal that HIR-dependent histone deposition represses the promoters of SAGA-dependent genes via antisense non-coding transcription. However, while antisense transcription is enriched at promoters of SAGA-dependent genes, this feature is not sufficient to define the mode of gene regulation. We further show that the balance between HIR-dependent nucleosome incorporation and transcription factor binding at promoters directs transcription into a SAGA- or TFIID-dependent regulation. This study sheds light on a new connection between antisense non-coding transcription and the nature of coding transcription initiation.
Databáze: OpenAIRE