Antisense-mediated repression of SAGA-dependent genes involves the HIR histone chaperone
Autor: | Julien Soudet, Nissrine Beyrouthy, Anna Marta Pastucha, Andrea Maffioletti, Dario Menéndez, Zahra Bakir, Françoise Stutz |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Nucleic Acids Research. 50:4515-4528 |
ISSN: | 1362-4962 0305-1048 |
DOI: | 10.1093/nar/gkac264 |
Popis: | Eukaryotic genomes are pervasively transcribed by RNA polymerase II (RNAPII), and transcription of long non-coding RNAs often overlaps with coding gene promoters. This might lead to coding gene repression in a process named Transcription Interference (TI). In Saccharomyces cerevisiae, TI is mainly driven by antisense non-coding transcription and occurs through re-shaping of promoter Nucleosome-Depleted Regions (NDRs). In this study, we developed a genetic screen to identify new players involved in Antisense-Mediated Transcription Interference (AMTI). Among the candidates, we found the HIR histone chaperone complex known to be involved in de novo histone deposition. Using genome-wide approaches, we reveal that HIR-dependent histone deposition represses the promoters of SAGA-dependent genes via antisense non-coding transcription. However, while antisense transcription is enriched at promoters of SAGA-dependent genes, this feature is not sufficient to define the mode of gene regulation. We further show that the balance between HIR-dependent nucleosome incorporation and transcription factor binding at promoters directs transcription into a SAGA- or TFIID-dependent regulation. This study sheds light on a new connection between antisense non-coding transcription and the nature of coding transcription initiation. |
Databáze: | OpenAIRE |
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