Inhibitory effect of black cumin (Nigella sativa) seed essential oil on Fusobacterium nucleatum L-methionine-γ-lyase (L-methioninase) activity
| Autor: | Nobuhiro Hanada, Takatoshi Murata, Mamiko Yamashita, Koji Shibuya, Masaaki Okamoto, Mayu Miyanohara, Hidenobu Senpuku, Masao Ishikawa |
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| Rok vydání: | 2020 |
| Předmět: |
0211 other engineering and technologies
Mixed inhibition 02 engineering and technology Microbial Sensitivity Tests Microbiology law.invention 03 medical and health sciences chemistry.chemical_compound Methionine law Ammonia 021105 building & construction Genetics Benzoquinones Oils Volatile Food science Nigella sativa Molecular Biology Essential oil Thymoquinone 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Fusobacterium nucleatum Chemistry biology.organism_classification Lyase Recombinant Proteins Anti-Bacterial Agents Butyrates Carbon-Sulfur Lyases Enzyme Seeds Bacteria |
| Zdroj: | FEMS microbiology letters. 368(7) |
| ISSN: | 1574-6968 |
| Popis: | The enzyme L-methionine-γ-lyase is commonly found in a wide range of bacteria and catalyzes the α-elimination and γ-elimination of L-methionine to produce methyl mercaptan, α-ketobutyrate and ammonia. Black cumin seed essential oil (BC oil) reportedly exhibits deodorizing activity against methyl mercaptan. Therefore, we hypothesized that BC oil may also suppress methyl mercaptan production. In this study, we aimed to evaluate the inhibitory effect of BC oil on L-methionine-γ-lyase activity in Fusobacterium nucleatum. Recombinant L-methionine-γ-lyase was incubated under appropriate conditions with BC oil and its constituent thymoquinone. To analyze L-methionine-γ-lyase activity, α-ketobutyric acid and ammonia concentrations were determined. The concentrations of α-ketobutyric acid and ammonia were significantly decreased by 10 µg mL−1 of BC oil (P |
| Databáze: | OpenAIRE |
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