Molecular Detection ofLeishmaniaDNA in Wild-Caught Phlebotomine Sand Flies (Diptera: Psychodidae) From a Cave in the State of Minas Gerais, Brazil
| Autor: | Reginaldo Peçanha Brazil, Felipe Dutra Rêgo, J. D. Andrade Filho, Mariana Campos das Neves Farah Ramos, Ana Paula Lusardo de Almeida Zenóbio, Gustavo Mayr de Lima Carvalho |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030231 tropical medicine Zoology Polymerase Chain Reaction DNA sequencing 03 medical and health sciences 0302 clinical medicine Species Specificity DNA Ribosomal Spacer parasitic diseases Botany Animals Parasite hosting Psychodidae Internal transcribed spacer Leishmania General Veterinary biology Sequence Analysis DNA DNA Protozoan 030108 mycology & parasitology biology.organism_classification Cavernicola Caves Infectious Diseases Insect Science Female Parasitology Subgenus Lutzomyia Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Medical Entomology. 54:196-203 |
| ISSN: | 1938-2928 0022-2585 |
| DOI: | 10.1093/jme/tjw137 |
| Popis: | Leishmania spp. are distributed throughout the world, and different species are associated with varying degrees of disease severity. In Brazil, Leishmania transmission involves several species of phlebotomine sand flies that are closely associated with different parasites and reservoirs, and thereby giving rise to different transmission cycles. Infection occurs during the bloodmeals of sand flies obtained from a variety of wild and domestic animals, and sometimes from humans. The present study focused on detection of Leishmania DNA in phlebotomine sand flies from a cave in the state of Minas Gerais. Detection of Leishmania in female sand flies was performed with ITS1 PCR-RFLP (internal transcribed spacer 1) using HaeIII enzyme and genetic sequencing for SSUrRNA target. The survey of Leishmania DNA was carried out on 232 pools and the parasite DNA was detected in four: one pool of Lutzomyia cavernicola (Costa Lima, 1932), infected with Le. infantum (ITS1 PCR-RFLP), two pools of Evandromyia sallesi (Galvao & Coutinho, 1939), both infected with Leishmania braziliensis complex (SSUrRNA genetic sequencing analysis), and one pool of Sciopemyia sordellii (Shannon & Del Ponte, 1927), infected with subgenus Leishmania (SSUrRNA genetic sequencing analysis). The present study identified the species for Leishmania DNA detected in four pools of sand flies, all of which were captured inside the cave. These results represent the first molecular detection of Lu cavernicola with Le infantum DNA, Sc sordellii with subgenus Leishmania DNA, and Ev sallesi with Leishmania braziliensis complex DNA. The infection rate in females captured for this study was 0.17%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|