Structure of the Human Urokinase Receptor Gene and Its Similarity to CD59 and the Ly-6 Family
| Autor: | William F. Doe, Jeffrey J. Selhamer, Jinjun Dang, Lorin K. Johnson, Yao Wang |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary Molecular Sequence Data CD59 Antigens Receptors Cell Surface CD59 Biology Biochemistry Receptors Urokinase Plasminogen Activator Plasminogen Activators Exon Antigens CD Transcription (biology) Complementary DNA Gene expression Antigens Ly Humans Amino Acid Sequence skin and connective tissue diseases neoplasms Gene Genetics Membrane Glycoproteins Base Sequence Sequence Homology Amino Acid Intron Exons Molecular biology Introns biological factors Urokinase receptor enzymes and coenzymes (carbohydrates) biological phenomena cell phenomena and immunity |
| Zdroj: | European Journal of Biochemistry. 227:116-122 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | Urokinase plasminogen activator receptor (uPAR) gene expression has been implicated in many important biological processes including cell invasiveness and migration. The uPAR gene was cloned from a human genomic library by hybridization with a uPAR cDNA. The complete structure of the human uPAR gene, including a 21.23-kb transcription unit with 204 bp 5' and 239 bp 3' flanking sequences, was determined by comparison with the uPAR cDNA sequence. The uPAR gene is composed of seven exons and six introns. The seven exons of 101, 111, 144, 162, 135, 147 and 563 bp are separated by six introns of approximately 2.04, 2.62, 8.42, 0.906, 3.10 and 2.78 kb. Exons 1-7 encode 19, 37, 48, 54, 45, 49 and 83 amino acid residues, respectively. A CpG-rich island and sequences related to the transcription factors AP-1, AP-2, c-Jun and NF kappa B are present, but no potential TATA or CAAT boxes were found in the proximal 5' region of the uPAR gene. Comparison of the exon organization of the uPAR gene with that of human CD59 and murine Ly-6 reveals similarity to all three domains encoded by the uPAR exons (2 + 3), (4 + 5) and (6 + 7). These data enable elucidation of the mechanisms involved in regulation of the uPAR gene expression and provide further evidence that the uPAR gene belongs to the Ly-6 superfamily. |
| Databáze: | OpenAIRE |
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