Titin antibodies in 'seronegative' myasthenia gravis--A new role for an old antigen
| Autor: | Jiri Pitha, Güher Saruhan-Direskeneli, F. Hanisch, Eleni Zamba-Papanicolaou, Talma Brenner, Beata Szyluk, Siegfried Labeit, Socrates J. Tzartos, C. Casasnovas Pons, John Tzartos, Dittmar Labeit, Julius Bogomolovas, Nils Erik Gilhus, Pilar Martinez-Martinez, Anna Kostera-Pruszczyk, Christos Stergiou, Ivana Basta, Konstantinos Lazaridis, Anthony Behin, Hacer Durmus, Francesca Andreetta, Angelina H. Maniaol, Sonia Berrih-Aknin, Kleopas A. Kleopa, A. Vaknin, Amelia Evoli, Piotr Szczudlik, Renato Mantegazza, M. De Baets, Tassos C. Kyriakides, Tarek Sharshar, Carlo Antozzi, M. Jakubíkova, Vasiliki Zouvelou, Chantal M. E. Tallaksen, Stojan Peric, Feza Deymeer, Dragana Lavrnic, Mario Losen |
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| Přispěvatelé: | RS: MHeNs - R3 - Neuroscience, Psychiatrie & Neuropsychologie |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Seronegative Male Radioimmunoprecipitation Assay animal structures Titin International Cooperation Immunology Enzyme-Linked Immunosorbent Assay Serology 03 medical and health sciences 0302 clinical medicine Antigen Receptors Diagnosis Myasthenia Gravis Autoantibodies Myasthenia gravis Radioimmunoprecipitation assay Connectin Female Humans LDL-Receptor Related Proteins Receptor Protein-Tyrosine Kinases Receptors Cholinergic Immunology and Allergy Neurology Neurology (clinical) Medicine Myopathy Cholinergic Autoimmune disease biology business.industry Autoantibody musculoskeletal system medicine.disease 3. Good health Settore MED/26 - NEUROLOGIA 030104 developmental biology biology.protein Antibody medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroimmunology Journal of Neuroimmunology, 292, 108-115. Elsevier |
| ISSN: | 1872-8421 0165-5728 |
| Popis: | Myasthenia gravis (MG) is an autoimmune disease caused by antibodies targeting the neuromuscular junction of skeletal muscles. Triple-seronegative MG (tSN-MG, without detectable AChR, MuSK and LRP4 antibodies), which accounts for similar to 10% of MG patients, presents a serious gap in MG diagnosis and complicates differential diagnosis of similar disorders. Several AChR antibody positive patients (AChR-MG) also have antibodies against titin, usually detected by ELISA. We have developed a very sensitive radioimmunoprecipitation assay (RIPA) for titin antibodies, by which many previously negative samples were found positive, including several from tSN-MG patients. The validity of the RIPA results was confirmed by western blots. Using this RIPA we screened 667 MG sera from 13 countries; as expected, AChR-MG patients had the highest frequency of titin antibodies (40.9%), while MuSK-MG and LRP4-MG patients were positive in 14.6% and 16.4% respectively. Most importantly, 13.4% (50/372) of the tSN-MG patients were also titin antibody positive. None of the 121 healthy controls or the 90 myopathy patients, and only 3.6% (7/193) of other neurological disease patients were positive. We thus propose that the present titin antibody RIPA is a useful tool for serological MG diagnosis of tSN patients. |
| Databáze: | OpenAIRE |
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