Activation of bovine prothrombin as monitored by the clotting assay
| Autor: | Ong Tc, Sothy Sp |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Time Factors Phospholipid Biochemistry chemistry.chemical_compound Thrombin Prothrombinase Internal medicine medicine Animals Drug Interactions Phospholipids Initial rate Clotting factor Factor V Endocrinology Coagulation chemistry Time course Factor Xa Calcium Cattle Prothrombin Blood Coagulation Tests circulatory and respiratory physiology medicine.drug |
| Zdroj: | Biological chemistry Hoppe-Seyler. 371(1) |
| ISSN: | 0177-3593 |
| Popis: | The activation of bovine prothrombin was studied with highly purified clotting factors and using a coagulation assay developed to look at the initial rate of prothrombin conversion as well as the conversion rate over a time course of 75 min. Activation of prothrombin by factor Xa alone was slow. The rate of prothrombin conversion increased markedly with the addition of each of the accessory components Ca2+, phospholipid and bovine factor V, respectively. With the complete prothrombinase complex comprising factor Xa, Ca2+, phospholipid and factor V, the rate increase was about 22,000-fold higher compared to the action of factor Xa and Ca2+ on prothrombin alone. The rates of thrombin formation obtained with activated factor X1 were only about 70% the values obtained with factor X2. The rate of prothrombin activation and the difference between the activities of the activated factors X1 and X2 are discussed. |
| Databáze: | OpenAIRE |
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