Acute stress results in skin corticotropin-releasing hormone secretion, mast cell activation and vascular permeability, an effect mimicked by intradermal corticotropin-releasing hormone and inhibited by histamine-1 receptor antagonists
| Autor: | Theoharis C. Theoharides, Duraisamy Kempuraj, Man Huang, Pamela Esposito, William Boucher, Michael Lytinas |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Restraint Physical endocrine system medicine.medical_specialty Injections Intradermal Corticotropin-Releasing Hormone Immunology Vascular permeability Histamine Release Allergic inflammation Capillary Permeability Rats Sprague-Dawley Corticotropin-releasing hormone chemistry.chemical_compound Stress Physiological Internal medicine medicine Immunology and Allergy Animals Mast Cells Receptor Olopatadine Hydrochloride Skin Urocortin business.industry General Medicine Rats Endocrinology chemistry Acute Disease Histamine H1 Antagonists Phthalazines Corticotropin-releasing hormone secretion Calcium business hormones hormone substitutes and hormone antagonists Histamine Dibenzoxepins Hormone |
| Zdroj: | International archives of allergy and immunology. 130(3) |
| ISSN: | 1018-2438 |
| Popis: | Background: Mast cells play an important role in allergic inflammation by releasing vasoactive molecules, proteases and cytokines. Corticotropin-releasing hormone (CRH) and its structural analogue urocortin (Ucn) were shown to trigger skin mast cell activation and vascular permeability. We investigated the effect of acute stress on rat skin vascular permeability and CRH secretion, as well as the effect of intradermal CRH, and that of two histamine-1 receptor antagonists, azelastine and olopatadine, on vascular permeability. Methods: Rats were stressed by restraint and vascular permeability was assessed by extravasation of 99Tc-gluceptate, while mast cell activation was determined by skin rat mast cell protease-1 (RMCP-1) content. Skin CRH content was evaluated by ELISA. The effect of intradermal injection of CRH and Ucn, as well as that of two histamine-1 receptor antagonists, azelastine and olopatadine, was assessed by Evan’s blue extravasation. Purified rat peritoneal mast cells (RPMCs) were also pretreated with azelastine (24 µM) or olopatadine (133 µM) for 5 min before challenge with compound 48/80 (0.5 µg/ml) for 30 min. Histamine secretion was measured fluorometrically. Intracellular Ca2+ ions were evaluated in RPMCs loaded with calcium crimson and stimulated with compound 48/80. Results: Acute stress increased skin vascular permeability and CRH content, while it decreased RMCP-1. Intradermal injection of CRH or Ucn induced substantial Evan’s blue extravasation that was inhibited by pretreatment with azelastine (24 µM) and olopatadine (133 µM). Both antihistamines also inhibited histamine release and intracellular increase of Ca2+ ions from RPMCs stimulated by compound 48/80. Conclusions: These results indicate that acute stress increases skin CRH that can trigger mast cell-dependent vascular permeability, effects inhibited by certain histamine-1 receptor antagonists, possibly acting to reduce intracellular Ca2+ ion levels. |
| Databáze: | OpenAIRE |
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