In vitro antiviral efficacy of caffeic acid against canine distemper virus
| Autor: | Peng-Fei Yi, Ben-Dong Fu, Zhen-Jiang Yu, Hao-Ran Li, Chun-Lei Zhang, Lu-Yuan Peng, Zhen-Qiang Cui, Zong-Mei Wu, Hai-Qing Shen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors animal diseases viruses 030106 microbiology Biology Virus Replication Microbiology Antiviral Agents Virus 03 medical and health sciences chemistry.chemical_compound Inhibitory Concentration 50 Caffeic Acids Chlorocebus aethiops Ribavirin Caffeic acid medicine Animals Viral rna Distemper IC50 Distemper Virus Canine Vero Cells Dose-Response Relationship Drug Canine distemper virus diseases food and beverages medicine.disease Virology In vitro Drug Combinations 030104 developmental biology Infectious Diseases chemistry Vero cell RNA Viral |
| Zdroj: | Microbial pathogenesis. 110 |
| ISSN: | 1096-1208 |
| Popis: | Canine distemper (CD) is a highly contagious disease caused by the canine distemper virus (CDV), and mortality can be as high as 100%. However, there is no specific treatment for CD. In this study, the antiviral activity of the caffeic acid against CDV was evaluated in vitro. The results showed that the IC50 of the caffeic acid against CDV at 1 and 2 h post infection (PI) is 23.3 and 32.3 μg/mL, respectively. Consistently, at 1 and 2 h PI, the caffeic acid exhibited a reduced (23.3–57.0% and 37.2–38.1%) viral inhibitory effect in vero cells. Furthermore, the caffeic acid plus Ribavirin (RBV) has greater antiviral activity against CDV than the caffeic acid or RBV individually. In addition, the caffeic acid reduced the total viral RNA synthesis by 59–86% at 24-72 h. Therefore, our data provided the experimental evidence that the caffeic acid effectively inhibited CDV infection in vero cells, which may potentially be used to treat clinical disease associated with CDV infection. |
| Databáze: | OpenAIRE |
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