Tumour necrosis factor blockade did not prevent the increase of muscular muscle RING finger-1 and muscle atrophy F-box in arthritic rats
| Autor: | M.A. Villanúa, Ana Isabel Martín, Teresa Priego, Miriam Granado, Asunción López-Calderón |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Necrosis Ubiquitin-Protein Ligases Endocrinology Diabetes and Metabolism Muscle Proteins Arthritis Adipose tissue Inflammation Cachexia Tripartite Motif Proteins Endocrinology Internal medicine medicine Animals RNA Messenger Insulin-Like Growth Factor I Rats Wistar Muscle Skeletal SKP Cullin F-Box Protein Ligases Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha business.industry Skeletal muscle medicine.disease Arthritis Experimental Muscle atrophy Rats medicine.anatomical_structure Receptors Tumor Necrosis Factor Type I Tumor necrosis factor alpha medicine.symptom Insulin-Like Growth Factor Binding Protein 5 business |
| Zdroj: | Journal of Endocrinology. 191:319-326 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.1.06931 |
| Popis: | Chronic inflammation is associated with a decrease in body weight and cachexia, which is characterized by anorexia and skeletal muscle wasting. The expression of atrogens muscle RING finger-1 (MuRF-1) and muscle atrophy F-box (MAFbx) are increased in muscle atrophy and it is known that tumour necrosis factor (TNF) regulates skeletal muscle loss through TNF receptor p55 (TNFRI). The aim of this study was to examine the effect of polyethylene glycol linked to soluble TNFRI (PEG-sTNFRI) on gene expression of the atrogens MuRF-1 and MAFbx in skeletal muscle of arthritic rats. Rats were injected with Freund’s adjuvant and, 15 days later, arthritic and control rats were injected daily with PEG-sTNFRI (1 mg/kg, s.c.) or saline for 8 days. Arthritis decreased body weight gain, the weight of skeletal muscle and adipose mass. PEG-sTNFRI administration increased body weight gain and adipose mass of arthritic rats; however, it did not modify the skeletal muscle weight. The gene expression of TNF-α, MuRF1 and MAFbx, IGF-I and IGFBP-5 were increased in the skeletal muscle of arthritic rats, and the administration of PEG-sTNFRI did not modify these parameters. These data suggest that the anti-TNF agent PEG-sTNFRI did not prevent the increase in E3 ubiquitin-ligating enzymes, MuRF1 and MAFbx, gene expression in the skeletal muscle of arthritic rats. |
| Databáze: | OpenAIRE |
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