Polysaccharide of Black cumin ( Nigella sativa) modulates molecular signaling cascade of gastric ulcer pathogenesis
| Autor: | Shylaja M. Dharmesh, Harsha Mysore Rajagopal, Srikanta Belagihalli Manjegowda |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Galectin 3 ATPase Pharmacology Polysaccharide Biochemistry Pathogenesis 03 medical and health sciences Polysaccharides Structural Biology Gastric mucosa medicine Extracellular Animals Nigella sativa Stomach Ulcer Rats Wistar Prostaglandin E2 Molecular Biology Mitogen-Activated Protein Kinase 1 chemistry.chemical_classification Mitogen-Activated Protein Kinase 3 Dose-Response Relationship Drug 030102 biochemistry & molecular biology biology Chemistry Mucin Mucins General Medicine digestive system diseases Rats 030104 developmental biology medicine.anatomical_structure Carbohydrate Sequence Matrix Metalloproteinase 9 Cyclooxygenase 2 biology.protein Matrix Metalloproteinase 2 Signal transduction medicine.drug |
| Zdroj: | International Journal of Biological Macromolecules. 101:823-836 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2017.03.093 |
| Popis: | Gastric ulcer is a multi-step disease and healing requires a complex process including repair and re-architecture of gastric mucosa with the involvement of molecular events. Current study was designed to understand the gastric ulcer healing mechanism of rhamnogalacturonan-I type pectic polysaccharide of black cumin (BCPP) utilizing acetic acid induced gastric ulcers in rats. BCPP fed groups at 200mg/kg b.w. for 10days showed up to 85% healing of gastric ulcers with modulation of key molecular events involved in ulcer healing process such as increase in gastric mucin content, cyclooxygenase-2 (Cox-2) and prostaglandin E2 (PGE2). The increase in extracellular signal-regulated kinase-2 (ERK-2) indicated that, BCPP could induce PGE-2 synthesis by increasing ERK-2 mediated COX-2 activity. Increase in matrix metalloproteinase-2 (MMP-2) and decrease in MMP-9 levels in BCPP treated groups indicated differential regulation of MMP-2 and 9, an essential event required for gastric mucosal re-modulation. BCPP containing bound phenolics (26mg/g) might have also played a role in increasing speed and quality of ulcer healing by inhibiting H+, K+ -ATPase and decreasing free radical mediated oxidation and cellular damages. Overall, studies showed that the polysaccharide can mediate ulcer healing by modulating signaling pathways involved in either ulcer aggravation or healing process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect