Rat embryonic mast cells originate in the AGM
| Autor: | Elaine Zayas Marcelino da Silva, Carolina Nunes França, Michel F. Guiraldelli, Celiane Cardoso Carvalho, Constance Oliver, Vanina Danuza Toso, Maria Célia Jamur, Devandir Antonio de Souza |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Cellular differentiation Science Immune Cells Immunology CD34 Biology GLICOPROTEÍNAS Molecular Cell Biology medicine Animals Mast Cells Progenitor cell Gonads Aorta Embryonic Stem Cells Multidisciplinary Membrane Glycoproteins Immunomagnetic Separation Receptors IgE Allergy and Hypersensitivity Stem Cells Cell Differentiation Hematology Mast cell Hematopoietic Stem Cells Embryonic stem cell Cell biology Rats Receptors Complement Haematopoiesis medicine.anatomical_structure Liver Mesonephros Medicine Female Bone marrow Stem cell Cellular Types Research Article Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 3, p e57862 (2013) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1932-6203 |
| Popis: | Mast cells originate from pluripotent hematopoietic stem cells. Two mast cell specific antibodies, mAbsAA4 and BGD6, have previously been used to identify and study committed mast cell precursors (MCcps) in the bone marrow of adult mice and rats. However, the embryonic origin of MCcps is still not known. In the present study, we identified MCcps in rat embryos using these previously characterized mast cell specific antibodies. The MCcps were found in the AGM (aorta-gonad-mesonephros) region of rat embryos at E11.5. These cells were BGD6+, CD34(+), c-kit(+), CD13(+), FcεRI(-), AA4(-) CD40(-), and Thy-1(-). By PCR the cells contained message for the α and β subunits of FcεRI and mast cell specific proteases. In vitro, the MCcps differentiated into metachromatic mast cells. With age of gestation the percent of MCcps diminished while the percent of mast cell progenitors increased. An increased knowledge of the biology and embryonic origin of mast cells may contribute to a greater understanding of allergy, asthma, and other mast cell related diseases. |
| Databáze: | OpenAIRE |
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