Reduced zinc cytotoxicity following differentiation of neural stem/progenitor cells into neurons and glial cells is associated with upregulation of metallothioneins
| Autor: | Hideki Mori, Mayu Nishikawa, Masayuki Hara |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Health
Toxicology and Mutagenesis Cellular differentiation Biology Toxicology Mice Chlorides Neural Stem Cells Downregulation and upregulation medicine Animals Metallothionein Progenitor cell Cytotoxicity Gene Cells Cultured Neurons Pharmacology Dose-Response Relationship Drug Cell Differentiation General Medicine Molecular biology Metallothionein 3 Neural stem cell Up-Regulation medicine.anatomical_structure Gene Expression Regulation Zinc Compounds Neuroglia Biomarkers Astrocyte |
| Zdroj: | Environmental Toxicology and Pharmacology. 39:1170-1176 |
| ISSN: | 1382-6689 |
| Popis: | We investigated zinc cytotoxicity in mouse neural stem/progenitor cells (NSPCs) and their differentiated progeny (neuronal/glial cells) in correlation with expression of metallothionein (MT) gene. Differentiated cells were less sensitive than NSPCs to ZnCl 2 (IC 50 : 128 μM vs. 76 μM). Differentiation of immature NSPCs to the differentiated cells led to an increase in expression of MT family genes ( Mt1 , Mt2 , Mt3 , and Mt4 ). Zinc exposure induced a dose-dependent increase in expression level of Mt1 and that of Mt2 in both NSPCs and the differentiated cells. Our results showed that the reduced cytotoxicity of zinc associated with differentiation from NSPCs into their progeny was related to the upregulation of MTs. |
| Databáze: | OpenAIRE |
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