Effects of a cantaloupe melon extract/wheat gliadin biopolymer during aortic cross-clamping

Autor: Hubert Schelzig, Catherine Garrel, Sukru Oter, Maura Albicini, Uwe B. Brückner, Hendrik Bracht, Günter Speit, Jörn Sträter, Florian Simon, Peter Radermacher, Xavier Leverve, Ulrich Ehrmann, Balázs Hauser, Jochen Kick, Claus-Martin Muth
Přispěvatelé: Hamant, Sarah, Abteilung Thorax- und Gefässchirurgie, Universitätsklinikum Ulm - University Hospital of Ulm, Sektion Anästhesiologische Pathophysiologie und Verfahrensentwicklung, Semmelweis Egyetem, Aneszteziológiai és Intenzív Terápiás Klinika, Istituto di Anestesiologia e Rianimazione, Università degli Studi di Milano = University of Milan (UNIMI), Fizyoloji Anabilim Dali, Gülhane Askeri Tip Akademisi, Département de biologie intégrée, CHU Grenoble-Hôpital Michallon, Abteilung Pathologie, Sektion Chirurgische Forschung, Bioénergétique fondamentale et appliquée, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Abteilung Humangenetik, Università degli Studi di Milano [Milano] (UNIMI)
Rok vydání: 2007
Předmět:
Male
Swine
Apoptosis
030204 cardiovascular system & hematology
Critical Care and Intensive Care Medicine
medicine.disease_cause
Gliadin
chemistry.chemical_compound
0302 clinical medicine
Cucumis melo
MESH: Animals
MESH: Hyperbaric Oxygenation
Food science
MESH: In Situ Nick-End Labeling
MESH: Swine
MESH: Cucumis melo
chemistry.chemical_classification
Hyperbaric Oxygenation
0303 health sciences
MESH: Oxidative Stress
biology
Glutathione peroxidase
MESH: Gliadin
food and beverages
3. Good health
Catalase
Reperfusion Injury
Anesthesia
Female
Comet Assay
DNA damage
MESH: Plant Extracts
MESH: Comet Assay
Nitric oxide
03 medical and health sciences
Intensive care
In Situ Nick-End Labeling
medicine
Animals
030304 developmental biology
MESH: DNA Damage
Plant Extracts
business.industry
MESH: Apoptosis
MESH: Male
Comet assay
Oxidative Stress
chemistry
biology.protein
MESH: Reperfusion Injury
business
MESH: Female
Oxidative stress
DNA Damage
Zdroj: Intensive Care Medicine
Intensive Care Medicine, Springer Verlag, 2007, 33 (4), pp.694-702. ⟨10.1007/s00134-006-0518-6⟩
ISSN: 1432-1238
0342-4642
DOI: 10.1007/s00134-006-0518-6
Popis: International audience; OBJECTIVE: We previously reported in healthy volunteers that a cantaloupe melon extract chemically combined with wheat gliadin (melon extract/gliadin) and containing SOD, catalase and residual glutathione peroxidase (GPx), protected against DNA strand-break damage induced by hyperbaric oxygen (HBO), a well-established model of DNA damage resulting from oxidative stress. Aortic cross-clamping is a typical example of ischemia/reperfusion injury-related oxidative stress, and therefore we investigated whether this melon extract/gliadin would also reduce DNA damage after aortic cross-clamping and reperfusion. DESIGN: Prospective, randomized, controlled experimental study. SETTING: Animal laboratory. PATIENTS AND PARTICIPANTS: 18 anesthetized, mechanically ventilated and instrumented pigs. INTERVENTIONS: After 14 days of oral administration of 1250 mg of the melon extract/gliadin (n=9) or vehicle (n=9), animals underwent 30 min of thoracic aortic cross-clamping and 4 h of reperfusion. MEASUREMENTS AND RESULTS: Before clamping, immediately before declamping, and at 2 and 4 h of reperfusion, we measured blood isoprostane (immunoassay) and malondialdehyde concentrations (fluorimetric thiobarbituric acid test), SOD, catalase and GPx activities (spectrophotometric kits), NO formation (nitrate+nitrite; chemoluminescence), DNA damage in whole blood samples and isolated lymphocytes exposed to hyperbaric oxygen (comet assay). Organ function was also evaluated. Kidney and spinal cord specimen were analysed for apoptosis (TUNEL assay). The melon extract/gliadin blunted the DNA damage, reduced spinal cord apoptosis and attenuated NO release, however, without any effect on lipid peroxidation and organ function. CONCLUSIONS: Pre-treatment with the oral melon extract/gliadin may be a therapeutic option to reduce oxidative cell injury affiliated with aortic cross-clamping.
Databáze: OpenAIRE
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