Hepatitis delta virus (HDV) and woodchuck hepatitis virus (WHV) nucleic acids in tissues of HDV-infected chronic WHV carrier woodchucks
| Autor: | Francesco Negro, B Forzani, John L. Gerin, T L Brown, Antonio Ponzetto, Brent E. Korba, B M Baroudy |
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| Rok vydání: | 1989 |
| Předmět: |
viruses
Immunology Biology Virus Replication Microbiology Virus chemistry.chemical_compound Liver Neoplasms Experimental Virology Hepatitis Viruses Animals Antigens Viral Woodchuck hepatitis virus Nucleic Acid Hybridization Sciuridae virus diseases RNA biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology digestive system diseases Liver Viral replication chemistry Hepadnaviridae Rolling circle replication Hepatitis Viral Animal Marmota Insect Science Carrier State DNA Viral Nucleic acid RNA Viral Hepatitis Delta Virus DNA Research Article |
| Zdroj: | Journal of Virology. 63:1612-1618 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.63.4.1612-1618.1989 |
| Popis: | The molecular forms of genomic and antigenomic hepatitis delta virus (HDV) RNA and of woodchuck hepatitis virus (WHV) DNA and WHV RNA were studied in nonneoplastic liver (NL) tissues, hepatocellular carcinoma (HCC) tissues, and several extrahepatic tissues of chronic WHV carrier woodchucks acutely (two animals) and chronically (six animals) superinfected with HDV. HDV was shown to replicate in all NL and HCC tissues but not in any of the extrahepatic tissues analyzed, which included spleen, peripheral blood lymphocytes, kidney, ovary, testis, thymus, lung, and stomach. HDV RNA was present as species with molecular weights consistent with those of monomers, dimers, and trimers of both strand polarities, supporting the rolling circle model proposed for HDV RNA replication. WHV DNA levels in NL, HCC, spleens, and serum were 10- to 100-fold lower than the levels typically observed in chronic WHV carrier woodchucks not infected with HDV. WHV DNA replicative intermediates were rarely observed and only at very low levels, representing less than 10% of the total WHV DNA. By contrast, WHV RNA transcription was not significantly depressed and both primary WHV RNA transcripts, 2.3 and 3.6 kilobases, were observed in NL, HCC, spleens, and in one of the kidney tissues. In addition, a 2.6-kilobase WHV RNA transcript was found in the majority of the NL tissues. |
| Databáze: | OpenAIRE |
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