Norovirus 3rd Generation kit: An improvement for rapid diagnosis of sporadic gastroenteritis cases and valuable for outbreak detection
| Autor: | Simone Guadagnucci Morillo, Maria do Carmo Sampaio Tavares Timenetsky, Adriana Luchs, Cibele Daniel Ribeiro, Audrey Cilli, Samira Julien Calux, Rita de Cássia Compagnoli Carmona |
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| Rok vydání: | 2011 |
| Předmět: |
Molecular Epidemiology
biology Clinical Laboratory Techniques business.industry Norovirus Outbreak biology.organism_classification medicine.disease_cause Sensitivity and Specificity Virology Caliciviridae Gastroenteritis Molecular Diagnostic Techniques medicine Humans Reagent Kits Diagnostic business Brazil Caliciviridae Infections Mixed infection |
| Zdroj: | Journal of Virological Methods. 173:13-16 |
| ISSN: | 0166-0934 |
| Popis: | The aim of this study was to evaluate the sensitivity and specificity of the Ridascreen® Norovirus 3rd Generation kit compared to the RT-PCR. A retrospective, descriptive study was conducted with 245 specimens from sporadic cases and outbreak surveillance samples of gastroenteritis in Brazil from 2006 to 2009. Overall, the kit showed a sensitivity of 61.8% and a specificity of 92.5%. The sensitivity for outbreaks diagnosis was 87.9% and specificity 83.8%. The Ridascreen® 3rd Generation could detect specimen containing genogroup (G) II with high sensitivity. However, GI and mixed infections (GI/GII) were unlikely to be detected by the kit. ELISA for Norovirus (NoV) detection provides a rapid, technically simple assay system that can be used to increase the surveillance of gastroenteritis outbreaks, especially in Public Health Laboratories with high sample throughput. This assay is useful for the detection of NoV outbreaks and is an improvement as compared to previous ELISAs; however, due to its sensitivity, RT-PCR in still required for routine NoV detection in sporadic cases investigation. |
| Databáze: | OpenAIRE |
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