The efficacy of delayed oxygen therapy in the treatment of experimental retinal detachment
| Autor: | Robert L. Avery, Steven K. Fisher, Kenneth A. Linberg, Kevin C. Talaga, Geoffrey P. Lewis |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Time Factors genetic structures Cell Survival Outer plexiform layer Cell Count Hyperoxia Muscle hypertrophy Ophthalmology Glial Fibrillary Acidic Protein In Situ Nick-End Labeling medicine Animals Vimentin Gliosis Eye Proteins Retina Microscopy Confocal CATS business.industry Oxygen Inhalation Therapy Retinal Detachment Retinal detachment Hypertrophy Anatomy medicine.disease Disease Models Animal medicine.anatomical_structure Cats sense organs medicine.symptom business Neuroglia Muller glia Cell Division Photoreceptor Cells Vertebrate Retinopathy |
| Zdroj: | American Journal of Ophthalmology. 137:1085-1095 |
| ISSN: | 0002-9394 |
| DOI: | 10.1016/j.ajo.2004.01.045 |
| Popis: | Purpose To evaluate the ability of delayed hyperoxia to slow or prevent degenerative and gliotic changes initiated by retinal detachment. Design An experimental study. Methods Rhegmatogenous detachments were produced in the right eyes of eight cats. After 1 day in room air (21% O 2 ), four cats were placed in chambers with the O 2 concentration regulated at 70%; the other four were left in room air. At 7 days the retinas were harvested and examined by light and confocal microscopy. Cell specific antibodies, TUNEL and proliferation assays, outer segment length, and photoreceptor counts, were used to assess the condition of the retina. The contralateral unoperated eyes were used as controls. Results Animals maintained in elevated O 2 showed a dramatic preservation of rod and cone outer segments as well as in the organization of the outer plexiform layer. The number of surviving photoreceptors was increased in the hyperoxia-treated animals. Neurite sprouting, a characteristic of detached retina, was rarely observed in the experimental eyes. Proliferation of non-neuronal cells was reduced, but not halted, by hyperoxia. GFAP and vimentin expression was not effected by hyperoxia; these intermediate filament proteins increased in Muller cells similar to that observed in control detachments. Conclusions Exposure to hyperoxia, delayed by 1 day after the onset of retinal detachment, was highly effective in preserving photoreceptor cells and in reducing proliferation within the retina. It did not, however, reduce the hypertrophy of Muller glia. There were no apparent detrimental effects of exposure to 70% O 2 for 6 days. These results suggest that human patients may benefit from breathing elevated oxygen levels while awaiting reattachment surgery, even if the hyperoxia is delayed relative to the time of detachment. |
| Databáze: | OpenAIRE |
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