miR-181a–Twist1 pathway in the chemoresistance of tongue squamous cell carcinoma
| Autor: | Bin Zhang, Jingsong Hou, Jian-guang Wang, Mo Liu, Anxun Wang, Hongzhang Huang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Epithelial-Mesenchymal Transition
animal structures Tongue squamous cell carcinoma Biophysics Antineoplastic Agents Biology Biochemistry Downregulation and upregulation microRNA Humans Epithelial–mesenchymal transition Molecular Biology Gene knockdown Twist-Related Protein 1 Nuclear Proteins Cell Biology Anatomy In vitro Tongue Neoplasms MicroRNAs Drug Resistance Neoplasm Cell culture Gene Knockdown Techniques Carcinoma Squamous Cell Cancer research Cisplatin Target gene Metabolic Networks and Pathways |
| Zdroj: | Biochemical and Biophysical Research Communications. 441:364-370 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2013.10.051 |
| Popis: | Although many researches have been undertaken to disclose the mechanisms of chemoresistance, the mechanisms remain unclear. The aim of this study is to elucidate the role of miR-181a-Twist1 pathway in the chemoresistance of tongue squamous cell carcinoma (TSCC). We found that cisplatin-induced chemoresistance in TSCC cell lines underwent EMT (epithelial-mesenchymal transition) and was accompanied by enhancing metastatic potential (migration and invasion in vitro), miR-181a downregulation and Twist1 upregulation. Functional analyses indicated that miR-181a reversed chemoresistance, inhibited EMT and metastatic potential in TSCC cells. Twist1 was confirmed as a direct miR-181a target gene by luciferase reporter gene assays. Twist1 knockdown by siRNA led to a reversal of the chemoresistance, inhibited EMT and metastatic potential in TSCC cells. Our study demonstrates that miR-181a-Twist1 pathway may play an important role in the development of cisplatin-chemoresistance, with EMT and an increase the metastatic potential of TSCC cells. |
| Databáze: | OpenAIRE |
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